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首页> 外文期刊>Archives of Toxicology >Mercuric dichloride induces DNA damage in human salivary gland tissue cells and lymphocytes.
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Mercuric dichloride induces DNA damage in human salivary gland tissue cells and lymphocytes.

机译：二氯化汞在人唾液腺组织细胞和淋巴细胞中引起DNA损伤。

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Amalgam is still one of the most frequently used dental filling materials. However, the possible adverse effects especially that of the mercuric component have led to continued controversy. Considering that mercury may be released from amalgam fillings into the oral cavity and also reach the circulating blood after absorption and resorption, it eventually may contribute to tumorigenesis in a variety of target cells. The present investigation focuses on genotoxic effects below a cytotoxic dose level of mercuric dichloride (HgCl(2)) in human samples of salivary glands and lymphocytes to elucidate a possible role in tumor initiation. DNA migration due to single strand breaks, alkali labile sites and incomplete excision repair was quantified with the aid of the single cell microgel electrophoresis (Comet) assay. The concepts of Olive Tail Moment, percentage of DNA in the Tail and Tail Length were used as measures of DNA damage. To control for cytotoxic effects, the trypan blue exclusion test was applied. Human samples of the parotid salivary gland and lymphocytes of ten donors were exposed to HgCl(2 )concentrations from 1 to 50 muM. N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and dimethyl sulfoxide (DMSO) served as controls. Increasing dose-dependent DNA migration could be demonstrated after exposure to HgCl(2) in cells of the salivary glands and lymphocytes. In both cell types a significant increase in DNA migration could be shown starting from HgCl(2 )concentrations of 5 muM in comparison to the negative control. The viability of the cell systems was not affected except at the highest concentration (50 muM) tested. These data indicate genotoxic effects of mercuric dichloride in human salivary glands and lymphocytes at concentrations not leading to cytotoxic effects or cell death. Consequently, a contributory role in oral salivary gland tumor initiation warrants further investigation.
机译：汞齐仍然是最常用的牙科填充材料之一。然而，可能的不利影响，特别是汞成分的不利影响，导致了持续的争议。考虑到汞可能从汞齐填充物释放到口腔中，并且在吸收和吸收后也到达循环血液，因此汞最终可能有助于多种靶细胞发生肿瘤。本研究的重点是在唾液腺和淋巴细胞的人类样品中，在低于细胞毒性剂量的二氯化汞（HgCl（2））的细胞毒性剂量水平下进行遗传毒性作用，以阐明在肿瘤起始中的可能作用。借助于单细胞微凝胶电泳（Comet）测定法对由于单链断裂，碱不稳定位点和不完全切除修复引起的DNA迁移进行了定量。橄榄尾矩的概念，尾巴中DNA的百分比和尾巴长度被用作DNA损伤的量度。为了控制细胞毒性作用，应用了锥虫蓝排斥试验。十名捐献者的腮腺唾液腺和淋巴细胞的人类样品暴露于HgCl（2）浓度从1到50μM。 N-甲基-N'-硝基-N-亚硝基胍（MNNG）和二甲基亚砜（DMSO）用作对照。唾液腺和淋巴细胞中的HgCl（2）暴露后，可以证明剂量依赖性DNA迁移增加。在两种细胞类型中，从HgCl（2）浓度为5μM的HgCl（2）开始，与阴性对照相比，DNA迁移都显着增加。除了在最高测试浓度（50μM）下，细胞系统的生存能力没有受到影响。这些数据表明二氯化汞在人类唾液腺和淋巴细胞中的遗传毒性作用不会导致细胞毒性作用或细胞死亡。因此，在口腔唾液腺肿瘤引发中的重要作用值得进一步研究。

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