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首页> 外文期刊>Asian-Australasian Journal of Animal Sciences >Differential Expression of PPAR gamma, FASN, and ACADM Genes in Various Adipose Tissues and Longissimus dorsi Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle
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Differential Expression of PPAR gamma, FASN, and ACADM Genes in Various Adipose Tissues and Longissimus dorsi Muscle from Yanbian Yellow Cattle and Yan Yellow Cattle

机译：延边黄牛和Yan黄牛各种脂肪组织和背阔肌中PPARγ，FASN和ACADM基因的差异表达

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摘要

The objective of this study was to investigate the correlation between cattle breeds and deposit of adipose tissues in different positions and the gene expressions of peroxisome proliferator-activated receptor gamma (PPAR gamma), fatty acid synthase (FASN), and Acyl-CoA dehydrogenase (ACADM), which are associated with lipid metabolism and are valuable for understanding the physiology in fat depot and meat quality. Yanbian yellow cattle and Yan yellow cattle reared under the same conditions display different fat proportions in the carcass. To understand this difference, the expression of PPAR gamma, FASN, and ACADM in different adipose tissues and longissimus dorsi muscle (LD) in these two breeds were analyzed using the Real-time quantitative polymerase chain reaction method (qRT-PCR). The result showed that PPAR gamma gene expression was significantly higher in adipose tissue than in LD in both breeds. PPAR gamma expression was also higher in abdominal fat, in perirenal fat than in the subcutaneous fat (p<0.05) in Yanbian yellow cattle, and was significantly higher in subcutaneous fat in Yan yellow cattle than that in Yanbian yellow cattle. On the other hand, FASN mRNA expression levels in subcutaneous fat and abdominal fat in Yan yellow cattle were significantly higher than that in Yanbian yellow cattle. Interestingly, ACADM gene shows greater fold changes in LD than in adipose tissues in Yan yellow cattle. Furthermore, the expressions of these three genes in lung, colon, kidney, liver and heart of Yanbian yellow cattle and Yan yellow cattle were also investigated. The results showed that the highest expression levels of PPAR gamma and FASN genes were detected in the lung in both breeds. The expression of ACADM gene in kidney and liver were higher than that in other organs in Yanbian yellow cattle, the comparison was not statistically significant in Yan yellow cattle.
机译：这项研究的目的是调查牛的品种与脂肪组织在不同位置的沉积之间的相关性以及过氧化物酶体增殖物激活受体γ（PPARγ），脂肪酸合酶（FASN）和酰基辅酶A脱氢酶（ ACADM），与脂质代谢有关，对于了解脂肪库和肉质的生理学非常有价值。在相同条件下饲养的延边黄牛和延边黄牛的the体脂肪比例不同。为了了解这种差异，使用实时定量聚合酶链反应法（qRT-PCR）分析了这两个品种的不同脂肪组织和背最长肌（LD）中PPARγ，FASN和ACADM的表达。结果表明，两个品种的脂肪组织中PPARγ基因的表达均显着高于LD。延边黄牛的腹部脂肪，肾周脂肪中的PPARγ表达也高于皮下脂肪（p <0.05），而延边黄牛的皮下脂肪中PPARγ表达显着高于延边黄牛。另一方面，Yan黄牛皮下脂肪和腹脂中FASN mRNA的表达水平明显高于Yan边黄牛。有趣的是，在Yan黄牛中，ACADM基因在LD中的折叠变化大于在脂肪组织中的折叠。此外，还研究了这三个基因在延边黄牛和延边黄牛的肺，结肠，肾，肝和心脏中的表达。结果表明，两个品种的肺中均检测到最高的PPARγ和FASN基因表达水平。延边黄牛肾脏和肝脏中ACADM基因的表达高于其他器官，而延黄黄牛中ACADM基因的表达没有统计学意义。

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