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首页> 外文期刊>Breeding science >An LMW-s glutenin gene of a hard red winter wheat is similar to an LMW-s gene of a Canadian Western Extra-Strong wheat.
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An LMW-s glutenin gene of a hard red winter wheat is similar to an LMW-s gene of a Canadian Western Extra-Strong wheat.

机译：硬红冬小麦的LMW-s谷蛋白基因类似于加拿大西部特强小麦的LMW-s基因。

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摘要

Experiments were conducted on wheat cultivars and lines to determine the relationship of KS2, KS3 and HS1 to GL1, GL2 and HA1, respectively, using SDS-PAGE and 2D-PAGE, and compared the gene structure corresponding to the low-molecular-weight glutenin subunits (LMW-GSs). KS2, KS3a and HS1 were resolved similarly to GL1, GL2 and HA1, respectively, in SDS-PAGE and 2D-PAGE. The sequences of the candidate gene for HS1 (HORO1) and the candidate gene for HA1 (HARU48K) coincided and the candidate gene for KS2/KS3a (KANS2) and the candidate gene for GL1/GL2 (GLEN42K) showed a very high identity with a difference of only one nucleotide. Although the allelism test was not performed either for HS1 and HA1 or for KS2/KS3a and GL1/GL2 in the present study, it was shown that KS2/KS3a and GL1/GL2 are allelic forms to HS1 and HA1, respectively. Furthermore, the sequences of the coding regions of the LMW-GS genes on each homologous group A chromosome (1A, 1B and 1D) displayed more than 96% identity and the identity of those among 1A, 1B and 1D was at most 96%. It is therefore suggested that KANS2, HORO1, GLEN42K and HARU48K are alleles at the same homologous Glu-3 locus. The results suggest that KS2, KS3a and HS1 are highly similar molecules to GL1, GL2 and HA1, respectively, not only on the basis of proteins but also on the basis of genes. A CWES spring wheat cultivar and a strong winter wheat line harboured highly similar LMW-s components associated with good bread making quality. A set of primer, s-F1/s-R2, amplified partial inner sequences of both KANS2 and GLEN42K associated with good bread making quality of a winter wheat line and a spring wheat cultivar, respectively..
机译：用SDS-PAGE和2D-PAGE分别对小麦品种和品系进行了实验，以确定KS2，KS3和HS1与GL1，GL2和HA1的关系，并比较了与低分子量谷蛋白对应的基因结构。亚单位（LMW-GSs）。在SDS-PAGE和2D-PAGE中，分别与GL1，GL2和HA1相似地解析了KS2，KS3a和HS1。 HS1的候选基因（HORO1）和HA1的候选基因（HARU48K）的序列重合，KS2 / KS3a的候选基因（KANS2）和GL1 / GL2的候选基因（GLEN42K）与A1的同源性很高。只有一个核苷酸的差异。尽管在本研究中未对HS1和HA1或KS2 / KS3a和GL1 / GL2进行等位基因检测，但已证明KS2 / KS3a和GL1 / GL2分别是HS1和HA1的等位基因形式。此外，每个同源A组染色体（1A，1B和1D）上的LMW-GS基因的编码区的序列显示出超过96％的同一性，并且在1A，1B和1D之间的那些的同一性至多为96％。因此，建议KANS2，HORO1，GLEN42K和HARU48K是在相同同源Glu-3基因座上的等位基因。结果表明，不仅基于蛋白质而且基于基因，KS2，KS3a和HS1分别与GL1，GL2和HA1是高度相似的分子。 CWES春季小麦品种和强壮的冬小麦品系具有与面包制作质量相关的高度相似的LMW-s成分。一组引物s-F1 / s-R2分别扩增了KANS2和GLEN42K的部分内部序列，分别与冬小麦品系和春小麦品种的良好制面包质量有关。

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