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首页> 外文期刊>Biocontrol Science >Simultaneous enrichment detection method for four types of pathogenic bacteria in food.
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Simultaneous enrichment detection method for four types of pathogenic bacteria in food.

机译：食品中四种致病菌的同时富集检测方法。

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摘要

Recently, the incidence of foodborne disease outbreaks associated with contaminated meat, seafood and fresh produce has increased worldwide. Enterohaemorrhagic Escherichia coli (EHEC) O157:H7, Salmonella spp., Listeria monocytogenes and Shigella spp. have been responsible for recent outbreaks associated with the consumption of such contaminated food. Hence, suitable detection methods for monitoring the risk of microbial food contamination are necessary. These bacterial pathogens in food could be detected by enrichment culture in buffered peptone water (BPW) and multiplex PCR analysis of culture broth. After the detection of pathogens by multiplex PCR analysis, each pathogen-positive sample was cultured in an enriched selective broth. After the selective enrichment culture, the culture broth was plated on a selective agar plate to obtain colonies. EHEC (>=13 cells/25 g), S. enteritidis (>=15 cells/25 g), L. monocytogenes (>=394 cells/25 g) and S. sonnei (>=2.4 cells/25 g) were detected and isolated. These pathogenic bacteria in frozen foods were also detected and isolated..
机译：最近，与污染的肉类，海鲜和新鲜农产品有关的食源性疾病暴发的发生率在世界范围内有所增加。肠出血性大肠杆菌（EHEC）O157：H7，沙门氏菌，单核细胞增生性李斯特菌和志贺氏菌。造成与食用此类受污染食品有关的近期暴发的原因。因此，需要用于监测微生物食物污染风险的合适的检测方法。食品中的这些细菌病原体可以通过在缓冲蛋白ept水（BPW）中进行富集培养并对培养液进行多重PCR分析来检测。在通过多重PCR分析检测病原体之后，将每个病原体阳性样品在富集的选择性肉汤中培养。选择性富集培养后，将培养液铺在选择性琼脂平板上以获得菌落。 EHEC（> = 13细胞/ 25 g），肠炎链球菌（> = 15细胞/ 25 g），单核细胞增生李斯特氏菌（> = 394细胞/ 25 g）和索氏链球菌（> = 2.4细胞/ 25 g）检测并隔离。还检测并分离了冷冻食品中的这些致病细菌。

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