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首页> 外文期刊>Biocontrol Science >Characterization of Microorganisms Isolated from the Black Dirt of Toilet Bowls and Componential Analysis of the Black Dirt
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Characterization of Microorganisms Isolated from the Black Dirt of Toilet Bowls and Componential Analysis of the Black Dirt

机译：马桶黑色污垢中分离出的微生物的表征及黑色污垢的成分分析

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We have previously conducted a microflora analysis and examined the biofilm-forming activity of bacteria isolated from toilet bowl biofilms. In the present investigation, to reveal the strain involved in the formation of black dirt in toilet bowls, we performed a microflora analysis of the bacteria and fungi isolated from the black dirt of toilet bowls at ten homes. Among samples from different isolation sites and sampling seasons, although a similar tendency was not seen in bacterial microflora, Exophiala sp was detected in the fungal microflora from all samples of black dirt except for one, and constituted the major presence. By scanning electron microscope (SEM) analysis of the formed black dirt, SEM image at X 1,000 and X 5,000 magnification showed objects like hyphae and many bacteria adhering to them, respectively. Micro fourier transform infrared spectroscopy (micro FT-IR) and SEM with X-ray microanalysis (SEM-XMA) were used to investigate the components of black dirt. IR spectra of micro-FT-IA showed typical absorptions associated with amide compounds and protein, and the elements such as C, N, 0, Na, Mg, Al, Si, P, S, K, and Ba were detected with SEM-XMA. These results showed that black dirt had living body ingredients. Furthermore, Exophiala sp. and Cladosporium sp. strains, which were observed at a high frequency, accumulated 2-hydroxyjuglone (2-HJ) and flaviolin as one of the intermediates in the melanin biosynthetic pathway by the addition of a melanin synthesis inhibitor (tricyclazole) at the time of cultivation. These results suggested strongly that the pigment of black dirt in toilet bowls was melanin produced by Exophiala sp. and Cladosporium sp. strains.
机译：我们先前进行了菌群分析，并检查了从抽水马桶生物膜分离出的细菌的生物膜形成活性。在本研究中，为了揭示与马桶中的黑色污垢形成有关的菌株，我们对十个家中从马桶中的黑色污垢中分离出的细菌和真菌进行了菌群分析。在来自不同分离点和取样季节的样品中，尽管在细菌菌群中未观察到相似的趋势，但在真菌菌群中，除一种菌外，所有黑色污垢样品均在真菌菌群中检出了Exophiala sp，并且主要存在。通过对形成的黑色污垢进行扫描电子显微镜（SEM）分析，放大倍数为X 1,000和X 5,000的SEM图像分别显示了菌丝等物体和粘附在其上的许多细菌。显微傅里叶变换红外光谱（micro FT-IR）和SEM和X射线微分析（SEM-XMA）用于研究黑色污垢的成分。 micro-FT-IA的红外光谱显示出典型的与酰胺化合物和蛋白质有关的吸收，并且使用SEM-可以检测到C，N，0，Na，Mg，Al，Si，P，S，K和Ba等元素XMA。这些结果表明黑色污垢具有活体成分。此外，Exophiala sp。和Cladosporium sp。高频观察到的菌株通过在培养时添加黑色素合成抑制剂（三环唑），在黑色素生物合成途径中积累了2-羟基juglone（2-HJ）和黄酮作为黑色素生物合成途径的中间体之一。这些结果强烈表明，马桶中黑色污垢的色素是Exophiala sp。生产的黑色素。和Cladosporium sp。株。

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