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Effect of alcohols on escherichia coil phages

机译：酒精对大肠杆菌噬菌体的影响

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Escherichia coli T even phages were ethanol sensitive,whereas T odd phages were ethanol tolerant.The phagecidal activities of alcohols on ethanol sensitive phages (T even,#lambda#) were revealed to be in the order n-propanol>ethanol>methanoln-butanoln-hexanoln-octanol.However,the those of alcohols on ethanol tolerant phages (T odd)were revealed to be in the order methanol>ethanol>n-propanol.The phagecidal activity of n-butanol (hydrophobic) increased when small amounts of hydrophilic methanol(10 and 20%,v/v),ethanol (10 and 20%,v/v) were added.The relative soluvbility curves of glucose plus Sudan III (hydrophobicity-hydrophilicity indication) were similar to the #lambda# phagecidal activity curves of ethanol solution.the denaturation of ethanol-sensitive phage proteins with ethanol solution (50 or 70%,v/v)on SDS-polyacrylamide gel electrophoresis was associated with a loss of the plaque-forming activity of the phage.these phenomena were not observed for the ethanoltolerant phages treated with ethanol solutions.The head proteins of ethanol-tolerant phage have a greater number of hydrophobic amino acids and fewer hydrophilic amino acids,whereas those of ethanol sensitive phage have a greater number of hydrophilic amino acids and fewer hydrophobic amino acids.From these data,we suggest that the hydrophobicity-hydrophilicity balance of the alcohol solution and the phage surface proteins affects the loss of the plaque-forming activity of E.coli phages by alcohol.
机译：大肠杆菌T偶噬菌体对乙醇敏感，而T奇噬菌体对乙醇敏感。醇对乙醇敏感噬菌体（Teven，＃lambda＃）的杀藻活性显示为正丙醇>乙醇>甲醇正丁醇正己醇正辛醇。但是，在耐乙醇的噬菌体（T奇数）上发现醇的顺序为甲醇>乙醇>正丙醇。正丁醇的杀菌活性当添加少量的亲水性甲醇（10％和20％，v / v），乙醇（10％和20％，v / v）时（疏水性）增加。葡萄糖和苏丹III的相对溶解度曲线（疏水性-亲水性指示）与乙醇溶液的＃lambda＃噬菌体活性曲线相似。在SDS-聚丙烯酰胺凝胶电泳上用乙醇溶液（50％或70％，v / v）变性乙醇敏感的噬菌体蛋白与噬菌斑的损失有关。噬菌体的形成活性。耐乙醇的噬菌体未观察到这些现象耐乙醇的噬菌体的头部蛋白具有大量的疏水氨基酸和较少的亲水性氨基酸，而乙醇敏感的噬菌体的头部蛋白具有更多的亲水氨基酸和较少的疏水氨基酸。因此，我们认为酒精溶液和噬菌体表面蛋白的疏水性-亲水性平衡会影响酒精对大肠杆菌噬菌体形成噬菌斑活性的损失。

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