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首页> 外文期刊>Biodegradation >Biodegradation of hexazinone by two isolated bacterial strains (WFX-1 and WFX-2).
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Biodegradation of hexazinone by two isolated bacterial strains (WFX-1 and WFX-2).

机译:两种分离的细菌菌株(WFX-1和WFX-2)对六嗪酮的生物降解作用。

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摘要

Two hexazinone-degrading bacterial strains were isolated from soil by enrichment culture technique, and identified as Pseudomonas sp. and Enterobacter cloacap, respectively. The two purified isolates, designated as WFX-1 and WFX-2, could rapidly degrade hexazinone with half-lives of 3.08 and 2.95 days in mineral salts medium (hereafter referred to as MSM). In contrast, their mixed bacterial culture (herein abbreviated as MBC) was found to degrade hexazinone, at an initial concentration of 50 mg l(-1), by enhancing 2.3-fold over that when the isolates were used alone. The degradation of hexazinone by MBC in MSM clearly decreased concomitant with the increase of initial concentration, and the level of hexazinone that was toxic enough to totally inhibit degradation was in the range of 150-200 mg l(-1). The appropriately combined conditions for hexazinone degradation by MBC in MSM were studied, and found to be pH 5.5, 30 degrees C and at agitation of 120 rpm. The addition of MBC to soil had a greater impact on disappearance of hexazinone, which nearly increased fivefold over that of the control set. As a result, findings in the present investigation provide useful information for soil and water decontamination of hexazinone.
机译:通过富集培养技术从土壤中分离出两株降解六嗪酮的细菌菌株,鉴定为假单胞菌。和Enterobacter cloacap。两种纯化的分离物,分别称为WFX-1和WFX-2,可以在矿物盐培养基(以下称为MSM)中以3.08和2.95天的半衰期快速降解六嗪酮。相反,发现它们的混合细菌培养物(在此缩写为MBC)在初始浓度为50 mg l(-1)时比单独使用分离物时提高2.3倍,从而降解了六嗪酮。 MBC在MSM中对六嗪酮的降解明显随初始浓度的增加而降低,而足以完全抑制降解的六嗪酮的水平在150-200 mg l(-1)的范围内。研究了在MSM中MBC降解六嗪酮的适当组合条件,发现其pH值为5.5,30摄氏度,搅拌速度为120 rpm。向土壤中添加MBC对六嗪酮的消失影响更大,与对照组相比几乎增加了五倍。结果,本研究中的发现为六嗪酮的土壤和水净化提供了有用的信息。

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