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首页> 外文期刊>Animal Genetics >Variant discovery in a QTL region on chromosome 3 associated with fatness in chickens
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Variant discovery in a QTL region on chromosome 3 associated with fatness in chickens

机译：在3号染色体上的QTL区域中发现与鸡脂肪相关的变异

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摘要

Abdominal fat content is an economically important trait in commercially bred chickens. Although many quantitative trait loci (QTL) related to fat deposition have been detected, the resolution for these regions is low and functional variants are still unknown. The current study was conducted aiming at increasing resolution for a region previously shown to have a QTL associated with fat deposition, to detect novel variants from this region and to annotate those variants to delineate potentially functional ones as candidates for future studies. To achieve this, 18 chickens from a parental generation used in a reciprocal cross between broiler and layer lines were sequenced using the Illumina next-generation platform with an initial coverage of 18X/chicken. The discovery of genetic variants was performed in a QTL region located on chromosome 3 between microsatellite markers LEI0161 and ADL0371 (33595706-42632651bp). A total of 136054 unique SNPs and 15496 unique INDELs were detected in this region, and after quality filtering, 123985 SNPs and 11298 INDELs were retained. Of these variants, 386 SNPs and 15 INDELs were located in coding regions of genes related to important metabolic pathways. Loss-of-function variants were identified in several genes, and six of those, namely LOC771163, EGLN1, GNPAT, FAM120B, THBS2 and GGPS1, were related to fat deposition. Therefore, these loss-of-function variants are candidate mutations for conducting further studies on this important trait in chickens.
机译：腹部脂肪含量是商业饲养鸡的经济上重要的特征。尽管已检测到许多与脂肪沉积相关的定量性状位点（QTL），但这些区域的分辨率较低，功能变体仍然未知。进行当前研究的目的是提高以前显示具有与脂肪沉积相关的QTL的区域的分辨率，以检测该区域的新变异，并注释这些变异以描绘潜在功能的变异，以作为未来研究的候选对象。为了实现这一目标，使用Illumina下一代平台对18羽来自肉代和产蛋鸡之间的亲本杂交的父母代鸡进行了测序，初始覆盖率为18X /只鸡。遗传变异的发现是在位于微卫星标记LEI0161和ADL0371（33595706-42632651bp）之间的3号染色体上的QTL区域中进行的。在该区域共检测到136054个SNP和15496个INDEL，经过质量过滤后，保留了123985个SNP和11298个INDEL。在这些变异中，有386个SNP和15个INDEL位于重要代谢途径相关基因的编码区域。在几个基因中鉴定出功能丧失的变异体，其中的六个即LOC771163，EGLN1，GNPAT，FAM120B，THBS2和GGPS1与脂肪沉积有关。因此，这些功能丧失的变体是候选突变，可用于进一步研究鸡的这一重要性状。

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