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首页> 外文期刊>Biocontrol Science and Technology >Isolation and characterisation of Aspergillus awamori BS05, a root-knot-nematode-trapping fungus
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Isolation and characterisation of Aspergillus awamori BS05, a root-knot-nematode-trapping fungus

机译：捕获根结线虫的真菌泡盛曲霉BS05的分离和鉴定

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摘要

Nematode-trapping fungi are important biocontrol agents against parasitic nematodes through adhesive or mechanical hyphal traps. Aspergillus awamori, a root-knot-nematode-trapping fungus from tomato rhizosphere soil, was identified based on morphology and molecular characteristics of internal transcribed spacer DNA sequence. Conidial heads were white to black brown, loosely globose, and 72-127 mu m in diameter. Conidiophores usually arose from the foot cell of basal mycelium, straight, and 960-1730 x 10.2-13.4 mu m, hyaline to pale brown, not constricted below the vesicles; vesicles hemispherical to elongate, 43-56 mu m in diameter, black brown, fertile over the upper half to two-thirds. Aspergilla were biseriate, and metulae were variable, 12-26 x 3.8-4.7 mu m; phialides were 8.2-9.4 x 2.5-3 mu m. Conidia were globose or subglobose, 3.6-4.8 mu m in diameter, rough, grey brown and parallel in chains. A. awamori BS05 showed 44.9% control efficacy against Meloidogyne incogtina in pot experiments which suggests it as a potential biocontrol agent against Meloidogyne. This is the first report on A. awamori as nematode-trapping fungus.
机译：捕获线虫的真菌是重要的生物防治剂，可通过粘合剂或机械菌丝捕获器来防治寄生线虫。根据内部转录间隔区DNA序列的形态和分子特征，鉴定了来自番茄根际土壤的根结线虫诱集真菌泡盛曲霉。分生孢子头白色至黑褐色，松散球形，直径72-127微米。分生孢子通常起源于基部菌丝的足细胞，直的，透明质的为960-1730 x 10.2-13.4μm，透明至浅棕色，不局限于小泡以下。囊泡半球形到拉长，直径43-56微米，黑褐色，在上半部至三分之二肥沃。曲霉菌为biseriate，而metulae为可变菌，为12-26 x 3.8-4.7μm。 phialides为8.2-9.4 x 2.5-3微米。分生孢子为球状或近球状，直径3.6-4.8微米，粗糙，灰棕色，链条平行。在盆栽实验中，泡盛曲霉BS05显示出对南方根结线虫的44.9％的防治效果，这表明它是潜在的根结线虫生物防治剂。这是关于泡盛曲霉作为捕线虫的真菌的首次报道。

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