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Suspension concentrate formulation of Bacillus thuringiensis var. kurstaki for effective management of Helicoverpa armigera on sunflower (Helianthus annuus)

机译:苏云金芽孢杆菌变种的悬浮浓缩制剂。有效地管理向日葵上的棉铃虫的kurstaki(菊苣)

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摘要

A local strain DOR Bt-1 belonging to Bacillus thuringiensis var. kurstaki (Bt. k) was multiplied through solid state fermentation and the resulting technical powder was milled and sieved to obtain particles of various sizes. Efficacy of Bt. k. against larvae of Helicoverpa armigera was found to increase with the decrease in particle size. Boric acid was found to be synergistic to DOR Bt-1 technical powder. LC50 of the Bt and boric acid mixture (75: 25) was lower at 89.63 mg/100 mL in comparison to Bt alone at 116.75 mg/100 mL. A suspension concentrate formulation with DOR Bt-1 technical was developed using boric acid as an adjuvant. The formulation was found to be highly effective against H. armigera in laboratory bioassays with an LC50 of 185 mu L (containing 53.36 mg Bt). The formulation gave effective control of H. armigera on sunflower within 3 days after spray even at the lowest dose of 1.0 mL/L under field conditions.
机译:苏云金芽孢杆菌变种的本地菌株DOR Bt-1。通过固态发酵将kurstaki(Bt。k)繁殖,将所得工业粉末进行研磨和筛分,以获得各种尺寸的颗粒。 Bt的功效。 k。发现对棉铃虫幼虫的抵抗力随着粒径的减小而增加。发现硼酸与DOR Bt-1工业粉末具有协同作用。与仅Bt的116.75 mg / 100 mL相比,Bt和硼酸混合物(75:25)的LC50较低,为89.63 mg / 100 mL。使用硼酸作为佐剂开发了具有DOR Bt-1技术的悬浮剂。在实验室生物测定中发现该制剂对棉铃虫具有高度有效的LC50为185μL(含53.36 mg Bt)。即使在田间条件下,即使在最低剂量为1.0 mL / L的情况下,喷雾后三天内,该制剂仍可有效控制向日葵上的棉铃虫。

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