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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Adventitious bud development and regeneration in Tillandsia eizii
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Adventitious bud development and regeneration in Tillandsia eizii

机译：铁兰的不定芽的发育与再生

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摘要

The bromeliad Tillandsia eizii is a striking species with large, colorful, and persistent inflorescences that can reach 1 m in length. The value of this plant as an ornamental and its importance in cultural and religious activities has led to its over-collection in the wild. Clonal propagation via tissue culture may be a means to repopulate native stands while meeting the demands for this species as an ornamental and ceremonial plant. Adventitious bud proliferation was induced from axenically germinated seedling material. Parameters evaluated were the age of explant material at the time of transfer onto bud-induction medium, the concentration of plant growth regulators, and the period of exposure to induction medium. Light and scanning electron microscopy (SEM) established the origin and development of buds. Twelve-week-old seedling explants rapidly initiated adventitious buds after a 30-d induction period on shoot-initiation medium. Adventitious buds were induced in 40% of the explants placed on media with 2 mg l(-1) 6-benzylaminopurine (BA) (8.88 mu M) plus 0.1 mg l(-1) alpha-naphthaleneacetic acid (NAA) (0.54 mu M) with some cultures becoming highly prolific after repeated subculture. Shoots elongated in proliferating cultures, and plants were successfully acclimatized and planted into the greenhouse. The results indicate that tissue culture may be used as a means to propagate this epiphytic bromeliad species, which is being seriously affected by deforestation and habitat destruction. In addition, adventitious bud proliferation can provide a means to propagate superior genotypes.
机译：凤梨铁兰（Tillandsia eizii）是一种引人注目的物种，其大花序，五颜六色和持续的花序可达1 m。这种植物作为观赏植物的价值及其在文化和宗教活动中的重要性，导致其在野外的收藏过多。通过组织培养进行的无性繁殖可能是在满足该物种作为观赏植物和礼仪植物的需求的同时重新繁殖原生林的方法。由不定芽发芽的幼苗材料诱导不定芽增殖。评估的参数是外植材料在转移到芽诱导培养基上时的年龄，植物生长调节剂的浓度以及暴露于诱导培养基的时间。光和扫描电子显微镜（SEM）确定了芽的起源和发育。在芽起始培养基上诱导30天后，十二周大的幼苗外植体迅速引发了不定芽。在40％的外植体中诱导不定芽，这些外植体中含有2 mg l（-1）6-苄氨基嘌呤（BA）（8.88μM）加0.1 mg l（-1）α-萘乙酸（NAA）（0.54 mu M），某些文化在反复传代培养后变得高度繁殖。芽在增殖的培养物中伸长，并且植物成功地适应环境并种植到温室中。结果表明，组织培养可以用作繁殖这种附生凤梨科物种的手段，这种物种受到毁林和栖息地破坏的严重影响。另外，不定芽的增殖可以提供繁殖优良基因型的手段。

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