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首页> 外文期刊>Indian Journal of Experimental Biology >Production of alpha-galactosidase from Aspergillus foetidus MTCC 6322 by solid state fermentation and its application in soymilk hydrolysis
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Production of alpha-galactosidase from Aspergillus foetidus MTCC 6322 by solid state fermentation and its application in soymilk hydrolysis

机译：固态发酵法从曲霉MTCC 6322生产α-半乳糖苷酶及其在豆浆水解中的应用

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摘要

The production of alpha-galactosidase from the wild fungal strain Aspergillus foetidus MTCC 6322 using solid state fermentation (SSF), its characterization, and its efficacy in the hydrolysis of soymilk using response surface methodology were studied. The optimum conditions for production of alpha-galactosidase by SSF were: wheat bran (10 g), moisture content (64%), inoculum volume (1.0 mL; 6 x 10(7) spores/mL) with a yield of 4.1 x 10(3) units per gram dry substrate (U/gds) at 96 h. The enzyme showed optimum activity at pH 6.0, temperature 40 degrees C, pH stability between 5.0-8.0, and temperature stability between 30-40 degrees C. The enzyme was stable in the presence of trypsin, lipase, and collagenase and it showed susceptibility of the substrates such as raffinose, melibiose, guar gum and soymilk to hydrolysis in varying degrees. The optimized conditions for soymilk hydrolysis were: soymilk (10 mL) from defatted soybean meal (1.5%), alpha-galactosidase (0.15 UmL(-1)) at 30 degrees C, pH 6.0 and duration of 1 h.
机译：研究了使用固态发酵（SSF）从野生真菌菌株Asetgillus foetidus MTCC 6322生产α-半乳糖苷酶，其表征以及其利用响应面法在豆浆水解中的功效。 SSF生产α-半乳糖苷酶的最佳条件是：麦麸（10 g），水分（64％），接种量（1.0 mL; 6 x 10（7）孢子/ mL），产量为4.1 x 10 （3）单位：96克/克干基质（U / gds）。该酶在pH 6.0，温度40摄氏度，pH稳定性在5.0-8.0和30-40摄氏度之间均显示最佳活性。该酶在存在胰蛋白酶，脂肪酶和胶原酶的情况下稳定，并表现出易感性。棉子糖，蜜二糖，瓜尔豆胶和豆浆等底物会不同程度地水解。豆浆水解的最佳条件为：脱脂豆粕中的豆浆（10 mL）（1.5％），30℃，pH 6.0和1 h持续时间的α-半乳糖苷酶（0.15 UmL（-1））。

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