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首页> 外文期刊>Indian Journal of Poultry Science >Size variation in the introniM region of CHD-W and CHD-Z genes amongdomestic galliformes species
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Size variation in the introniM region of CHD-W and CHD-Z genes amongdomestic galliformes species

机译:国内鸡形目物种中CHD-W和CHD-Z基因内含子区域的大小变化

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摘要

The primers specific to CHD-W/Z sequence (P2 and P8) were used explore size variation in the intronic region of CHD-W and CHD-Z genes and utilize this variation in sex differentiation in chicken, guinea fowl, quail and turkey. In chicken, two bands (360 bp and 376 bp) were observed in female, while only one band (360 bp), was observed in male while in guinea fowl, turkey and quail only one band was observed in both the sexes. In guinea fowl and turkey, the size of this band was 360 bp, but in quail,it was 380 bp in size. Hence in the domestic gaUiformes species, the polymorphism in the intronic region of CHD-W and CHD-Z genes was quite evident, however, P2 and P8 primers, which were very effective in sex differentiation in chicken, cannot be used in the related gaUiformes species.
机译:使用对CHD-W / Z序列(P2和P8)具有特异性的引物来探索CHD-W和CHD-Z基因内含子区域的大小变异,并利用这种变异在鸡,珍珠鸡,鹌鹑和火鸡中进行性别分化。在鸡中,雌性观察到两个条带(360 bp和376 bp),而雄性仅观察到一个条带(360 bp),而在珍珠鸡,火鸡和鹌鹑中,两性仅观察到一个条带。在珍珠鸡和火鸡中,该条带的大小为360 bp,而在鹌鹑中,条带的大小为380 bp。因此,在国内鸭形目物种中,CHD-W和CHD-Z基因内含子区域的多态性非常明显,但是,在鸡的性别分化中非常有效的P2和P8引物不能用于相关的鸭形目中。种类。

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