Gene profiling of laser-microdissected brain regions and sub-regions.

Sanna PP; King AR; van der Stap LD; Repunte Canonigo V

首页> 外文期刊>Brain research. Brain research protocols >Gene profiling of laser-microdissected brain regions and sub-regions.

【24h】

Gene profiling of laser-microdissected brain regions and sub-regions.

机译：激光显微切割的大脑区域和子区域的基因图谱。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The application of transcriptomics and proteomics approaches to accurately dissected anatomically-defined brain regions and sub-regions remains a central focus of current neurobiological investigations as well as a necessary step towards single-neuron neurogenomics and neuroproteomics. A protocol is described for the simple, rapid, and reproducible laser microdissection of brain regions and sub-regions for microarray-based gene expression analyses from individual rats or mice using two rounds of in vitro transcription (IVT). The results presented also demonstrate that the current Affymetrix GeneChip arrays are well suited for this experimental design with high reproducibility and limited effects of the shortening of target RNA caused by the double IVT approach.

机译：转录组学和蛋白质组学方法在准确解剖解剖学定义的大脑区域和子区域中的应用仍然是当前神经生物学研究的重点，也是迈向单神经元神经基因组学和神经蛋白质组学的必要步骤。描述了一种协议，用于对大脑区域和子区域进行简单，快速和可重复的激光显微切割，以便使用两轮体外转录（IVT）从单个大鼠或小鼠中进行基于微阵列的基因表达分析。提出的结果还表明，当前的Affymetrix GeneChip阵列具有很高的可重复性，并且由于双重IVT方法而导致的靶RNA缩短的效果有限，因此非常适合该实验设计。

著录项

来源
《Brain research. Brain research protocols》 |2005年第2期|共9页
作者
Sanna PP; King AR; van der Stap LD; Repunte Canonigo V;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类神经病学与精神病学;
关键词
Brain; Gene Expression Profiling; Lasers; Microdissection; Oligonucleotide Array Sequence Analysis; 脑; 激光; 寡核苷酸序列分析;

机译：Brain;Gene Expression Profiling;Lasers;Microdissection;Oligonucleotide Array Sequence Analysis;脑;激光;寡核苷酸序列分析;

相似文献

外文文献
中文文献
专利

1. Gene profiling of laser-microdissected brain regions and sub-regions. [J] . Sanna PP, King AR, van der Stap LD, Brain research. Brain research protocols . 2005,第2期

机译：激光显微切割的大脑区域和子区域的基因图谱。
2. Angiogenic Gene Profiles in Laser-Microdissected Microvessels and Neurons from Ischemic Penumbra of Rat Brain [J] . Tian Yingfang, Di Yuanyuan, Zhang Jianshui, Journal of Molecular Neuroscience: MN . 2019,第4期

机译：激光 - 微小微小肌肌血管生成基因谱和大鼠缺血半霜的神经元
3. Brain Region-Specific Neurodegenerative Profiles Showing the Relative Importance of Amphetamine Dose, Hyperthermia, Seizures, and the Blood-Brain Barrier [J] . John F. Bowyer, Monzy Thomas, Larry C. Schmued, Annals of the New York Academy of Sciences . 2008,第期

机译：脑区域特定的神经退行性特征显示苯丙胺剂量，热疗，癫痫发作和血脑屏障的相对重要性
4. Computational neuroanatomy and gene expression: Optimal sets of marker genes for brain regions [C] . Grange Pascal, Mitra Partha P. Annual Conference on Information Sciences and Systems;CISS . 2012

机译：计算神经解剖学和基因表达：脑区域标记基因的最佳集合
5. Neurogenesis Is Altered between and across Brain Regions after Neurodegeneration and Injury [D] . Perez, Alice. 2019

机译：神经发生在神经变性和损伤后的脑区之间发生改变。
6. Different post-mortem brain regions from three Chinese FFI patients induce different reactive profiles both in the first and second generation RT-QuIC assays [O] . Kang Xiao, Qi Shi, Wei Zhou, 2020

机译：来自三种中国FFI患者的不同后验尸脑区均在第一代和第二代RT-Quic测定中诱导不同的反应性曲线
7. Figure 1: Example of visualization of long range contacts for: (A) regions, here enhancer-promoter contacts, for two 10 kb resolution Hi-C maps from human Fetal Brain cells. Contacts were calculated up to 30 bins distance each way from the point of interest. Top to bottom: a Jbrowse (Skinner et al., 2009) screenshot with genes and interaction profile representation (points of interest in green, their contact predictions in yellow); HiCEnterprise interaction profile plot with intensities (weighted by distance) and −log10 of FDR corrected p-values (q-values) with a threshold set at 0.01. (B) TADs, here HiCEnterprise visualization for 150 kb resolution Hi-C map of 17th chromosome from HUVEC. Left upper trianle: original Hi-C map contact frequencies; right lower triangle: −log10 of q-values of the interdomain interactions calculated with hypergeometric distribution. [O] . -1

机译：图1：用于：（a）区域的长距离触点的可视化示例，这里增强了来自人胎儿脑细胞的两个10kb分辨率的Hi-C地图。从兴趣点计算联系人高达30个距离距离。顶部到底：jbrowse（Skinner等，2009）截图，具有基因和互动曲线表示（绿色兴趣点，它们的黄色接触预测）; HICENTERPLISE交互配置曲线图具有强度（由距离加权）和FDR校正的P值（Q值）的-LOG10，其阈值设置为0.01。（b）TADS，这里是来自Huvec的第17次染色体的150 KB分辨率Hi-C地图的Hicenterprise可视化。左上三角：原版Hi-C地图接触频率;右下三角形：跨度相互作用的Q值的-log10计算，使用超几何分布计算。

Gene profiling of laser-microdissected brain regions and sub-regions.

摘要

著录项

相似文献

相关主题

期刊订阅