• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Brain research. Developmental brain research >Genotype-dependent sex differentiation of dopaminergic neurons in primary cultures of embryonic mouse brain.
【24h】

Genotype-dependent sex differentiation of dopaminergic neurons in primary cultures of embryonic mouse brain.

机译：多巴胺能神经元的基因型依赖性性别分化在胚胎小鼠大脑的原代培养中。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In order to investigate genetic factors that interfere with hormone-mediated sex differentiation of dopaminergic neurons, we raised sex-specific primary cultures from embryonic day 13 diencephalon (D) or mesencephalon (M) of three different strains of mice, NMRI, CBA/J, and BALBc/J. Part of the cultures were maintained for 6 or 13 days in vitro (DIV) in medium containing 17 beta-estradiol or testosterone. The cultures were analyzed for sex differences in numbers of tyrosine hydroxylase-immunoreactive neurons, endogenous dopamine (DA) levels, and specific uptake of [3H]DA. Previous results obtained with cultures of embryonic Sprague-Dawley rats had shown that these parameters develop sex-specific characteristics in the absence of sex differences in hormone environment. Similar steroid-independent sex differences as they occur in the rat were found in M cultures of NMRI but not in CBA and BALBc mice. Long-term sex steroid treatment did not affect any of the above parameters in any strain. It is concludedthat cell-autonomous realization of the genetic sex of dopaminergic neurons depends on the genetic background.
机译：为了研究干扰激素介导的多巴胺能神经元性别分化的遗传因素，我们从三种不同小鼠，NMRI，CBA / J的胚胎第13天双脑（D）或中脑（M）募集了具有性别特异性的原代培养物。 ，以及BALBc / J。在含有17个β-雌二醇或睾丸激素的培养基中，将部分培养物体外（DIV）维持6或13天。分析了培养物的酪氨酸羟化酶免疫反应性神经元数量，内源多巴胺（DA）水平和[3H] DA特异性摄取的性别差异。胚胎Sprague-Dawley大鼠培养物获得的先前结果表明，在激素环境中没有性别差异的情况下，这些参数会发展出性别特异性的特征。在NMRI的M培养物中发现了与在大鼠中相似的类固醇独立性别差异，但在CBA和BALBc小鼠中未发现。长期性激素治疗在任何菌株中均不影响上述任何参数。结论是，多巴胺能神经元遗传性别的细胞自主实现取决于遗传背景。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号