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首页> 外文期刊>Briefings in functional genomics & proteomics >iTRAQPak: an R based analysis and visualization package for 8-plex isobaric protein expression data
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iTRAQPak: an R based analysis and visualization package for 8-plex isobaric protein expression data

机译:iTRAQPak:基于R的8重等压蛋白质表达数据的分析和可视化软件包

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摘要

The field of high throughput proteomics has spawned a number of mass spectrometry-based technologies, which enable the quantitative analysis of protein expression. One of these technologies is iTRAQ (trademarked by Applied Biosystems), which through the use of isobaric tags, enables the quantitation of up to eight complex protein samples in a single multiplexed analysis. Isobaric tagging methods are emerging as an important tool to study protein expression dynamics. In this report, we describe iTRAQPak, a free software package developed in the R statistical and visualization environment that can be applied to the analysis of 8-plex expression data. The utility of this package is demonstrated through its application to the analysis of 8-plex iTRAQ protein expression data obtained from cerebrospinal fluid samples from Alzheimer's disease subjects involved in a Phase I drug trial.
机译:高通量蛋白质组学领域催生了许多基于质谱的技术,这些技术可以对蛋白质表达进行定量分析。这些技术之一就是iTRAQ(Applied Biosystems的商标),该技术通过使用同量异位标记,可以在一次多重分析中定量多达8种复杂的蛋白质样品。等压标记法正在成为研究蛋白质表达动力学的重要工具。在此报告中,我们描述了iTRAQPak,这是在R统计和可视化环境中开发的免费软件包,可用于分析8重表达数据。该软件包的实用性通过将其用于分析从I期药物试验涉及的阿尔茨海默氏病受试者的脑脊液样本中获得的8重iTRAQ蛋白表达数据而得到证明。

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