The use of a fluorescent bacteriophage assay for detection of Escherichia coli O157:H7 in inoculated ground beef and raw milk: bacteriophage has so many possibilities but is desperately under-exploited

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The use of a fluorescent bacteriophage assay for detection of Escherichia coli O157:H7 in inoculated ground beef and raw milk: bacteriophage has so many possibilities but is desperately under-exploited

机译：荧光噬菌体测定法用于检测接种的碎牛肉和生乳中的大肠杆菌O157：H7：噬菌体具有多种可能性，但极度开发不足

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The objective of this study was to develop a fluorescent bacteriophage assay (FBA) for the detection of E. coli O157:H7 in ground beef and raw milk. The FBA is a two step assay that combines immunomagnetic separation, to separate the target organism from mixed culture, with a highly specific fluorescently stained bacteriophage to label the E. coli O157:H7 cells.

机译：这项研究的目的是开发一种荧光噬菌体测定法（FBA），用于检测牛肉和生奶中的大肠杆菌O157：H7。 FBA是两步检测法，结合了免疫磁分离技术和高特异性荧光染色噬菌体，可将目标生物体从混合培养物中分离出来，以标记大肠杆菌O157：H7细胞。

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《International Food Safety News》 |1999年第6期|共1页
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正文语种 eng
中图分类营养卫生、食品卫生;
关键词
food safety; pot pourri;

机译：食品安全;锅装;

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1. The use of a fluorescent bacteriophage assay for detection of Escherichia coli O157:H7 in inoculated ground beef and raw milk: bacteriophage has so many possibilities but is desperately under-exploited [J] . International Food Safety News . 1999,第6期

机译：荧光噬菌体测定法用于检测接种的碎牛肉和生乳中的大肠杆菌O157：H7：噬菌体具有多种可能性，但极度开发不足
2. Development and Characterization of a Fluorescent-Bacteriophage Assay for Detection of Escherichia coli O157:H7 [J] . Lawrence Goodridge, Jinru Chen, Mansel Griffiths Applied and Environmental Microbiology . 1999,第4期

机译：荧光噬菌体检测大肠杆菌O157：H7的开发与表征
3. Comparative Evaluation of a Phage Protein Ligand Assay with Real-Time PCR and a Reference Method for the Detection of Escherichia coli O157:H7 in Raw Ground Beef and Trimmings [J] . F. SAVOYE, P. FENG, C. ROZAND, Journal of food protection . 2011,第1期

机译：实时PCR和参考方法对生牛肉和切碎大肠埃希菌O157：H7的噬菌体蛋白配体测定的比较评估
4. The Effects of a Three-strain Mixture of Bacteriophage on E. coli O157:H7-inoculated Hide Squares over a 10-hour Period [C] . Mies P.D., Davis M.J. ICoMST 2011;International conference of meat science and technology . 2012

机译：三株噬菌体混合物在10小时内对大肠杆菌O157：H7接种的兽皮的影响
5. Interactions between Escherichia coli O157:H7 and environmental protozoa: The role of protozoa in seasonal variation of E. coli O157:H7 on cattle farms and effects of Shiga toxin-encoding bacteriophages on protozoan grazing. [D] . Schmidt, Carrie E. 2015

机译：大肠杆菌O157：H7与环境原生动物之间的相互作用：原生动物在牛场大肠杆菌O157：H7季节性变化中的作用以及志贺毒素编码噬菌体对原生动物放牧的影响。
6. Development and Characterization of a Fluorescent-Bacteriophage Assay for Detection of Escherichia coli O157:H7 [O] . Lawrence Goodridge, Jinru Chen, Mansel Griffiths 1999

机译：荧光噬菌体检测大肠杆菌O157：H7的开发与表征
7. Detecção de Escherichia coli O157:H7 inoculada experimentalmente em amostras de leite cru por método convencional e PCR multiplex Detection of E. coli O157:H7 experimentally inoculated in raw milk samples by conventional method and multiplex PCR [O] . P.M. Garcia, E.F. Arcuri, M.A.V.P. Brito, 2008

机译：通过常规方法和多重pCR实验接种原料乳样品中的大肠杆菌O157：H7的检测通过常规方法和多重pCR实验接种在原料乳样品中的大肠杆菌O157：H7的检测
8. Risk-based Sampling for Escherichia coli O157:H7 in Ground Beef and Beef Trim [R] . 2008

机译：基于风险的大肠杆菌O157：H7在碎牛肉和牛肉切片中的取样

The use of a fluorescent bacteriophage assay for detection of Escherichia coli O157:H7 in inoculated ground beef and raw milk: bacteriophage has so many possibilities but is desperately under-exploited

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