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首页> 外文期刊>International Journal of Animal Sciences >Effect of protein and energy levels on rumen metabolites in deccani sheep
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Effect of protein and energy levels on rumen metabolites in deccani sheep

机译：蛋白质和能量水平对德卡尼羊瘤胃代谢产物的影响

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摘要

Thirty-six weaned male Deccani lambs weighing between 8.0 to 9.5 kg were randomly alloted to nine groups, namely, LPLE, LPME, LPHE, MPLE, MPME, MPHE, HPLE, HMPE and HPHE ill a 3 x 3 factorial design. The levels of protein and energy in concentrate mixtures were 80,100 and 120% of ICAR (1985) requirements and the medium level being 13% CP and 65% TDN To meet the requirements, concentrate mixture was fed at the rate of 350 and 450 g per animal per day during phase I (3 to 6 months of age) and phase II (6 to 12 months of age), respectively, with ad libitum mixed grass hay (mainly comprising Heteropogan contortus and Sehima nervosum). Rumen liquor was collected at 6 and 12 months of age at the end of each metabolism trial at 0 (before feeding), 2, 4 and 6 h after feeding. The strained rumen liquor was analysed for pH, TVFA and nitrogen fractions. The mean pH values of SRL were lowest (P<0.01) at 2 h post-feeding The rumen pH ranged from 6.28+0.02 to 6.58+-0.02 during phase I and 6. 17+-0.03 to 6.53+-0.02 during phase II. Tile peak concentrations of TVFA, total nitrogen, ammonia nitrogen, TCA insoluble nitrogen and residual nitrogen were attained at 2 h post-feeding. The mean concentration of TVFA, total nitrogen, ammonia nitrogen, TCA insoluble nitrogen, residual nitrogen and food and protozoal nitrogen varied from 23.39+-0.39 to 39.31+-06.9, 80.61+-0.74 to 91.11+-1.92, 7.41+-0.09 to 13.57+-0.30, 29.81+-0.39 to 39.11+-0.66, 17.29+-0.25 to 22.49+-0.38 and 24.08+-1.18 to 26.43+-1.00 during phase I and 23.47+-0.40 to 38.47+-0.75, 80.94+-0.90 to 99.31+-1.79, 7.91+-0.15 to 14.87+-0.39, 32.17+-0.42 to 39.83+-0.59, 18.17+-0.31 to 22.15+-0.34 and 22.53+-1.09 to 23.62+-0.96 during phase II, respectively. Tile rumen pH values decreased (P<0.05) as energy level of diet increased and there was insignificant difference in pH due to variation in dietary protein levels. Total VFA and ruminal bacterial nitrogen increased significantly (P<0.05) with increase in protein and energy levels and a reverse trend was observed with ruminal ammonia nitrogen.
机译：将三只断奶雄性Deccani羔羊体重在8.0至9.5 kg之间，随机分配给9组，分别为LPLE，LPME，LPHE，MPLE，MPME，MPHE，HPLE，HMPE和HPHE，分为3 x 3因子设计。浓缩混合物中蛋白质和能量的水平为ICAR（1985）要求的80,100和120％，中等水平为13％CP和65％TDN。为满足要求，浓缩混合物的进料速度为每吨350和450 g在I期（3至6个月大）和II期（6至12个月大）期间，动物每天随意采食混合草干草（主要包括变种Heteropogan contortus和Sehima nervosum）。在每个新陈代谢试验结束时，在0（喂食前），喂食后2、4和6小时，在6和12个月大时收集瘤胃液。分析滤过的瘤胃液的pH，TVFA和氮含量。喂食后2小时，SRL的平均pH值最低（P <0.01）。第一阶段的瘤胃pH范围为6.28 + 0.02至6.58 + -0.02，第二阶段的瘤胃pH范围为6 + 17--0.03至6.53 + -0.02 。进料后2 h达到TVFA，总氮，氨氮，TCA不溶氮和残留氮的峰值浓度。 TVFA，总氮，氨氮，TCA不溶氮，残留氮和食物以及原生动物氮的平均浓度在23.39 + -0.39至39.31 + -06.9、80.61 + -0.74至91.11 + -1.92、7.41 + -0.09至第I阶段的13.57 + -0.30、29.81 + -0.39至39.11 + -0.66、17.29 + -0.25至22.49 + -0.38和24.08 + -1.18至26.43 + -1.00和23.47 + -0.40至38.47 + -0.75、80.94 +第二阶段期间-0.90至99.31 + -1.79、7.91 + -0.15至14.87 + -0.39、32.17 + -0.42至39.83 + -0.59、18.17 + -0.31至22.15 + -0.34和22.53 + -1.09至23.62 + -0.96 ， 分别。日粮的瘤胃pH值随着日粮能量水平的增加而降低（P <0.05），并且由于日粮蛋白质水平的变化，pH值差异不显着。随着蛋白质和能量水平的增加，总VFA和瘤胃细菌氮显着增加（P <0.05），而瘤胃氨氮则观察到相反的趋势。

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