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首页> 外文期刊>International Chickpea and Pigeonpea Newsletter >An Easy Method for Isolating the Solanapyrone Toxins from Culture Filtrates of Ascochyta rabiei
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An Easy Method for Isolating the Solanapyrone Toxins from Culture Filtrates of Ascochyta rabiei

机译：从狂犬病壁球菌培养滤液中分离茄碱酮毒素的简便方法

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摘要

Blight, caused by Ascochyta rabiei, is one of the most serious diseases of chickpea (Nene 1982). The fungus attacks all aerial parts of the plant causing epinasty of petioles and young branches followed by water soaking and necrosis. These symptoms are consistent with the breakdown of the semipermeable properties of plasmamembranes of the plant. Using fluorescein diacetate, a dye which causes live cells with intact membranes to fluoresce under long wavelength UV light, culture filtrates of the pathogen were found to kill cells isolated enzymically from leaflets of the plant. The assay was used to monitor purification of the toxins. Three compounds were obtained and identified as solanapyrones A, B, and C (Alam et al. 1989, Latif et al. 1993, Chen and Strange 1991). The purpose of this report is to make available and speedy method for obtaining pure preparations of the toxins.
机译：由狂犬病Ascochyta rabiei引起的枯萎病是鹰嘴豆最严重的疾病之一（Nene 1982）。真菌侵袭植物的所有地上部分，引起叶柄和幼枝的蔓延，然后浸水和坏死。这些症状与植物的质膜的半透性破坏有关。使用荧光素二乙酸酯（一种使具有完整膜的活细胞在长波长紫外线下发光）的染料，发现病原体的培养滤液能杀死从植物小叶中酶分离的细胞。该测定法用于监测毒素的纯化。获得了三种化合物，并确定为茄那酮A，B和C（Alam等，1989; Latif等，1993; Chen和Strange，1991）。本报告的目的是提供一种可用于获得毒素纯制剂的快速方法。

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