...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >High molecular mass complexes of aquatic silk proteins
【24h】

High molecular mass complexes of aquatic silk proteins

机译：水生丝蛋白的高分子复合物

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Little is known about specific protein-protein associations that take place during formation of Chironomus tentans silk. The aim of this study was to learn if C. tentans salivary glands contain biochemically discrete silk protein complexes. Examination of native extracts by non-denaturing agarose gel electrophoresis and immunoblotting revealed two SDS-resistant complexes; C1a, nominally containing silk proteins spIa, sp185 and sp140, and C1b, containing spIb, sp185 and sp140. The data also implied that C1a and C1b can further associate into SDS-sensitive homo- or hetero-oligomers. Sedimentation of extracts in preparative glycerol gradients resulted in a heterogeneous distribution of C1a and C1b centered near 30S. Examination of gradient fractions by denaturing polyactrylamide gel electrophoresis and immunoblotting indicated that C1a and C1b co-sediment with spIs, sp185, and sp140; however, these fractions also contained sp40, sp17 and sp12. In contrast, two other silk proteins sedimented throughout the gradient. Electron micrographs of a complex-containing fraction showed discrete, sometimes oligomeric lattice-like structures that, over time, assembled in vitro into multistranded beaded fibers. It is proposed that C1a and C1b are quaternary structures that are intermediates in the assembly pathway of C. tentans silk.
机译：关于在田野猫（Chironomus tentans）丝形成过程中发生的特定蛋白质-蛋白质缔合知之甚少。这项研究的目的是要了解滕氏梭菌唾液腺是否含有生化离散的丝蛋白复合物。通过非变性琼脂糖凝胶电泳和免疫印迹检测天然提取物，发现了两种抗SDS的复合物。 C1a，名义上包含丝蛋白spIa，sp185和sp140，C1b，包含spIb，sp185和sp140。数据还暗示C1a和C1b可以进一步缔合为SDS敏感的均聚物或杂聚物。提取物在制备的甘油梯度中的沉淀作用导致C1a和C1b的分布不均匀，集中在30S附近。通过变性聚丙烯酰胺凝胶电泳和免疫印迹检查梯度级分，表明C1a和C1b与spIs，sp185和sp140共沉淀。但是，这些级分还包含sp40，sp17和sp12。相反，其他两种丝蛋白在整个梯度中沉积。含有配合物的馏分的电子显微照片显示出离散的，有时为低聚物的格子状结构，随着时间的推移，它们在体外组装成多股的串珠纤维。有人提出C1a和C1b是四级结构，它们是C.tentans丝的组装路径中的中间产物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号