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首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Multi-spectroscopic study on interaction of bovine serum albumin with lomefloxacin-copper(II) complex
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Multi-spectroscopic study on interaction of bovine serum albumin with lomefloxacin-copper(II) complex

机译：牛血清白蛋白与洛美沙星-铜（II）配合物相互作用的多光谱研究

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摘要

The binding reactions of lomefloxacin-copper(II) complex (LMF-Cu) or LMF to bovine serum albumin (BSA) in physiological solution were investigated by multi-spectroscopy. The binding constant, the number of binding sites and the binding distance between LMF-Cu or LMF and BSA were obtained by a fluorescence quenching method and according to the mechanism of Forster-type dipole-dipole non-radioactive energy-transfer, respectively. Enthalpy and entropy changes for two systems were calculated to be -7.970 kJ mol(-1) and 47.438 J mol(-1) K-1 for LMF-BSA, - 12.469 kJ mol(-1) and 33.542 J mol(-1) K-1 for LMF-Cu-BSA, respectively. The highly positive values observed for the entropy give evidence for a strong interaction. The values of Delta H and Delta S in two systems are similar, indicating that electrostatic interactions in two systems play major role. The effect of LMF-Cu or LMF on the conformation of BSA was also analyzed by synchronous fluorescence, three-dimensional fluorescence and circular dichroism spectra. The results showed that the presence of Cu ion in LMF-Cu can affect the conformation of BSA to some degree. All the results revealed that the addition of copper ion promotes the interaction of lomefloxacin with bovine serum albumin. (c) 2006 Elsevier B.V. All rights reserved.
机译：通过多光谱研究了洛美沙星-铜（II）复合物（LMF-Cu）或LMF与牛血清白蛋白（BSA）在生理溶液中的结合反应。通过荧光猝灭法并根据Forster型偶极-偶极非放射性能量转移机理，分别获得了LMF-Cu或LMF与BSA之间的结合常数，结合位点数和结合距离。对于LMF-BSA，两个系统的焓和熵变化计算为-7.970 kJ mol（-1）和47.438 J mol（-1）K-1，-12.469 kJ mol（-1）和33.542 J mol（-1 ）分别为LMF-Cu-BSA的K-1。观察到的熵的高度正值为强烈的相互作用提供了证据。两个系统中的Delta H和Delta S的值相似，表明两个系统中的静电相互作用起主要作用。还通过同步荧光，三维荧光和圆二色性光谱分析了LMF-Cu或LMF对BSA构象的影响。结果表明，LMF-Cu中Cu离子的存在可在一定程度上影响BSA的构象。所有结果表明，铜离子的添加​​促进洛美沙星与牛血清白蛋白的相互作用。 （c）2006 Elsevier B.V.保留所有权利。

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