• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Protein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure
【24h】

Protein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure

机译：肠上皮共培养物的蛋白表达谱：功能化碳纳米管暴露的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To assess the biological effects of low level, water dispersible, functionalised carbon nanotube (f-CNT) exposure in an in vitro model simulating the digestive tract, cellular protein expression was quantified and compared using label-free quantitative mass spectrometry (LFQMS). Co-cultured cells were exposed to well-characterised SWCNT-COOH, MWCNT-COOH, and MWCNT-PVP. The relative expression of 2,282 unique proteins was compared across the dose groups. 428 proteins were found to be differentially expressed. At the high dose, the extent of differential protein expression was CNT-specific and directly related to CNT colloidal stability. Cells responded to low level MWCNT-PVP exposure with three-fold greater differential expression. Bioinformatic analysis indicated significant and f-CNT-specific effects on relevant molecular and cellular functions and canonical pathways, with little overlap across f-CNT type and in the absence of overt toxicity.
机译：为了在体外模拟消化道的模型中评估低水平，水分散性，功能化碳纳米管（f-CNT）暴露的生物学效应，对细胞蛋白表达进行了定量和比较，使用了无标记定量质谱法（LFQMS）。将共培养的细胞暴露于特征明确的SWCNT-COOH，MWCNT-COOH和MWCNT-PVP。在各个剂量组之间比较了2,282种独特蛋白的相对表达。发现428种蛋白质被差异表达。在高剂量下，差异蛋白表达的程度是CNT特异性的，并且与CNT胶体稳定性直接相关。细胞对低水平的MWCNT-PVP暴露具有三倍的差异表达。生物信息学分析表明，f-CNT对相关分子和细胞功能以及规范途径具有显着且特定的作用，在f-CNT类型上几乎没有重叠，并且没有明显的毒性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号