• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Fabrication of bienzymatic cholesterol biosensor based on gold nanopartlcles decorated graphene-nanostructured polyaniline nanocomposite
【24h】

Fabrication of bienzymatic cholesterol biosensor based on gold nanopartlcles decorated graphene-nanostructured polyaniline nanocomposite

机译：金纳米粒子修饰的石墨烯-纳米结构聚苯胺纳米复合材料双酶胆固醇生物传感器的制备

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel amperometric cholesterol biosensor based on bienzyme system such as cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) was developed based on the gold nanoparticles decorated graphene-nanostructured polyaniline nanocomposite (NSPANI/AuNP/GR) film, electrochemically deposited onto indium-tin-oxide (ITO) electrode. The cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) have been coimmobilised onto the NSPANI/AuNP/GR nanocomposite electrode using gluteraldehyde as a crosslinking agent. The nanobioelectrodes ChOx-HRP/NSPANI/AuNP/GR/ITO and ChOx-/NSPANI/AuNP/GR/lTO have been characterised by differential pulse voltammetery (DPV), cyclic voltammetry (CV) and scanning electron microscopy (SEM). The bienzymatic nanobioelectrodes ChOx-HRP/NSPANI/AuNP/GR/ITO have exhibited higher sensitivity than mono enzymatic bioelectrode (ChOx-/NSPANI/AuNP/GR/ITO). Minimum interferences have been observed from ascorbic acid, uric acid, sodium pyruvate, glucose and urea for both single and bienzyme systems. It is inferred that bienzyme based nanobioelectrodes offer wider linearity (35 to 500 mg/dl), higher sensitivity (4.22 |jAmM-l), high shelf life (8 weeks), low response time (19s) and high accuracy for testing of blood serum samples than mono enzyme system. Mechanism of the overall biochemical reaction has been proposed to illustrate the enhanced bio-sensing performance of the bienzyme system.
机译：基于金纳米粒子修饰的石墨烯-纳米结构聚苯胺纳米复合材料（NSPANI / AuNP / GR）膜，电化学沉积在铟锡上，开发了一种基于双酶系统的新型安培胆固醇生物传感器，例如胆固醇氧化酶（ChOx）和辣根过氧化物酶（HRP）。氧化物（ITO）电极。已使用戊二醛作为交联剂将胆固醇氧化酶（ChOx）和辣根过氧化物酶（HRP）共固定在NSPANI / AuNP / GR纳米复合电极上。纳米生物电极ChOx-HRP / NSPANI / AuNP / GR / ITO和ChOx- / NSPANI / AuNP / GR / 1TO的特征在于差分脉冲伏安法（DPV），循环伏安法（CV）和扫描电子显微镜（SEM）。双酶纳米生物电极ChOx-HRP / NSPANI / AuNP / GR / ITO的敏感性高于单酶生物电极（ChOx- / NSPANI / AuNP / GR / ITO）。对于单酶和双酶系统，从抗坏血酸，尿酸，丙酮酸钠，葡萄糖和尿素中观察到的干扰最小。可以推断，基于双酶的纳米生物电极具有更宽的线性度（35至500 mg / dl），更高的灵敏度（4.22 | jAmM-1），高保存期限（8周），响应时间短（19s）以及血液检测的高精度血清标本比单酶系统高。已经提出了整个生化反应的机理来说明双酶系统的增强的生物传感性能。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号