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首页> 外文期刊>International Journal of Food Microbiology >Detection of shiga-toxin producing E-coli (STEC) in leafy greens sold at local retail markets in Alexandria, Egypt
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Detection of shiga-toxin producing E-coli (STEC) in leafy greens sold at local retail markets in Alexandria, Egypt

机译：在埃及亚历山大港当地零售市场上出售的绿叶蔬菜中检测产生志贺毒素的大肠杆菌（STEC）

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Leafy green vegetables, a popular and an indispensable ingredient of the daily menus of Egyptians' diets, currently presents a great concern in terms of microbiological hazards. To the best of our knowledge, this is the first report that provides scientific evidence for prevalence of shiga-toxigenic Escherichia coli (STEC) in leafy greens sold at open air local retail markets and superstores in the Egyptian environment. A total of 486 conventional and organic leafy green samples that are eaten raw were collected from different areas in Alexandria, evaluated for total E. coli counts (ECCs), and screened for E. coli O157:H7 using conventional and molecular methods. Recovery of E. coli (>= 10(2) CFU/g) from all studied types of leafy greens was indicative of fecal contamination. Total ECCs in conventional samples ranged from 5.47 to 2.56 log CFU/g. Based on their inability to ferment sorbitol on CT-SMAC media, 26 presumptive E. coli O157 isolates were detected in 71.4% (270/378) of the studied conventional samples. From all studied organic samples, only 2 types (organic cabbage and parsley, 16.7%) were contaminated with presumptive E. coli O157. All 28 isolates were further serotyped as E. coli O157 by latex agglutination test, and biochemically confirmed as E. coli. Multiplex PCR assays confirmed the ability of 21.4% (6/28) of the E. coli O157 strains to produce shiga-toxins (Stxs), and their virulence markers were as follows: stx(1), 66.6% (4/6); stx(2), 50% (3/6); stx(1)/stx(2), 16.7% (1/6); eaeA, 83.3% (5/6); and hlyA,16.7% (1/6). Only 2 strains recovered from conventional and organic parsley could possibly be classified as E. coli O157:H7 based on the presence of stx-genes (either stx(1) or stx2 or both). Results of the present research highlight that high E. coli loads, together with recovery of STEC O157 isolates could pose serious health risks to the produce consumers. This emphasizes the urgent need for health authorities to value and utilize the existing knowledge to identify strategies that reduce microbiological risks due to fecal contamination of agricultural products, and implement control measures at all stages of the food chain to specifically eliminate the presence of STEC O157 on the leafy green category. (C) 2014 Elsevier B.V. All rights reserved.
机译：绿叶蔬菜是埃及人日常饮食中一种流行且必不可少的成分，目前在微生物危害方面引起了极大关注。就我们所知，这是第一份报告，为埃及本地环境中的露天零售商店和大型超市出售的绿叶蔬菜中的滋生毒素的大肠杆菌（STEC）流行提供了科学依据。从亚历山德里亚的不同地区收集了总共486份未加工的常规和有机绿叶绿色样品，评估了大肠杆菌的总计数（ECC），并使用常规和分子方法筛选了O157：H7大肠杆菌。从所有研究类型的绿叶蔬菜中回收大肠杆菌（> = 10（2）CFU / g）表明粪便受到污染。常规样品中的总ECC范围为5.47至2.56 log CFU / g。由于无法在CT-SMAC培养基上发酵山梨糖醇，在71.4％（270/378）的常规样品中检出了26种推定的大肠杆菌O157分离株。在所有研究的有机样品中，仅2种类型的有机大白菜和欧芹（16.7％）被推定的大肠杆菌O157污染。通过乳胶凝集试验将所有28个分离株进一步血清分型为大肠杆菌O157，并通过生化确认为大肠杆菌。多重PCR分析证实21.4％（6/28）的大肠杆菌O157菌株产生志贺毒素（Stxs）的能力，其毒力标记如下：stx（1），66.6％（4/6） ; stx（2），50％（3/6）; stx（1）/ stx（2），16.7％（1/6）; eaeA，83.3％（5/6）;和hlyA，16.7％（1/6）。基于stx基因（stx（1）或stx2或两者）的存在，从常规欧芹和有机欧芹中回收的仅2株可能被分类为大肠杆菌O157：H7。本研究结果表明，高大肠杆菌含量以及STEC O157分离株的回收可能对农产品消费者构成严重的健康风险。这强调了卫生主管部门迫切需要重视和利用现有知识，以鉴定降低农产品粪便污染所致微生物风险的战略，并在食物链的各个阶段实施控制措施，以专门消除STEC O157的存在。绿叶类别。 （C）2014 Elsevier B.V.保留所有权利。

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