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Genetic similarity among Tunisian olive cultivars and two unknown feral olive trees estimated through SSR markers

机译：通过SSR标记估算突尼斯橄榄品种与两棵未知野生橄榄树之间的遗传相似性

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We used eight informative microsatellite markers for fingerprinting and evaluation of genetic similarity among 15 Tunisian olive (Olea europaea L.) cultivars and two feral unknown trees named Soulela 1 and Soulela 2. Thirty-one alleles were revealed, and the number of alleles per SSR varied from 2 (UDO12) to 6 (GAPU71A). Cluster analysis grouped cultivars into three main clusters. The two unknown varieties could not be reliably classified into any of these cultivar groups. SSR analysis indicated the presence of three erroneous denominations of cultivars. We resolved two synonymy cases (Zalmati and Chemlali; Rkhami and Chetoui) and one case of homonymy (Chemlali Tataouine). Genetic analyses of DNA extracted from leaves, oils, and embryos of the two unknown cultivars and the two major Tunisian olive cultivars (Chemlali and Chetoui) were also studied. We conclude that the reliable identification of these two feral cultivars needs to be addressed by a larger set of markers.
机译：我们使用了八个信息性微卫星标记，对15个突尼斯橄榄（Olea europaea L.）品种和两棵野生无名树Soulela 1和Soulela 2进行指纹鉴定和遗传相似性评估。揭示了31个等位基因，每个SSR的等位基因数量从2（UDO12）到6（GAPU71A）不等。聚类分析将品种分为三个主要类别。这两个未知品种无法可靠地分类为这些品种中的任何一个。 SSR分析表明存在三种错误的品种。我们解决了两个同义案例（Zalmati和Chemlali; Rkhami和Chetoui）和一个同义案例（Chemlali Tataouine）。还对从两个未知品种和两个主要突尼斯橄榄品种（Chemlali和Chetoui）的叶，油和胚中提取的DNA进行了遗传分析。我们得出结论，这两个野生品种的可靠鉴定需要通过更多的标记物解决。

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