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首页> 外文期刊>Biochemical Genetics >Isolation and expression of two distinct Sox8 genes in mudloach (Misgurnus anguillicaudatus).
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Isolation and expression of two distinct Sox8 genes in mudloach (Misgurnus anguillicaudatus).

机译：泥mud（Misgurnus anguillicaudatus）中两个不同的Sox8基因的分离和表达。

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摘要

To investigate the function and evolutionary origin of the SOXE subgroup, we amplified the genomic DNA of Misgurnus anguillicaudatus using a pair of degenerate primers. Using RACE, we obtained two versions of Sox8 (MaSox8a and MaSox8b) from M. anguillicaudatus. The overall sequence identity of the deduced amino acids from the two genes was 54.38%, with only one amino acid change in the high-mobility group domain. Southern blotting and evidence from the phylogenetic tree provided further proof for the existence of two Sox8 genes at the genomic level. This is the first evidence of two distinct Sox8 genes in Cypriniformes. Semi-quantitative and real-time quantitative PCR assays showed the expression trend of the genes was opposite in early embryonic development, and both were expressed ubiquitously in several adult tissues. The similar expression patterns indicated that MaSox8a and MaSox8b have possible overlapping functions.
机译：为了研究SOXE亚组的功能和进化起源，我们使用一对简并引物扩增了Misgurnus anguillicaudatus的基因组DNA。使用RACE，我们从M. anguillicaudatus获得了Sox8的两个版本（MaSox8a和MaSox8b）。从这两个基因推导的氨基酸的总体序列同一性为54.38％，而在高迁移率基团域中只有一个氨基酸改变。 Southern印迹和系统树的证据为基因组水平上两个Sox8基因的存在提供了进一步的证据。这是鲤形目中两个不同的Sox8基因的第一个证据。半定量和实时定量PCR分析表明，基因的表达趋势在早期胚胎发育中是相反的，并且都在几个成年组织中普遍表达。相似的表达模式表明MaSox8a和MaSox8b具有可能的重叠功能。

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