...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>International journal of immunogenetics >The polymorphism of human leucocyte antigen-G gene in a healthy population of Iran.
【24h】

The polymorphism of human leucocyte antigen-G gene in a healthy population of Iran.

机译：伊朗健康人群中人类白细胞抗原-G基因的多态性。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human leucocyte antigen-G (HLA-G) is a non-classical HLA class I molecule that unlike the classical HLA, has low polymorphism. This molecule, initially, found on invasive trophoblast cells and is postulated to have mediatory role in maternal-fetal interface. So far 43 alleles of HLA-G gene have been found. Studies on alleles of HLA-G gene could be useful in understanding the genetic variants of HLA-G alleles in Iranian population. The goal of this research was to determine the polymorphism of HLA-G gene in a healthy population of Iran. Genomic DNA was isolated from the whole blood of 102 randomly selected, healthy, unrelated Iranian individuals using salting-out technique followed by polymerase chain reaction (PCR) amplification of the exons 2 and 3 of HLA-G gene. For the performance of PCR-restriction fragment length polymorphism method, the PCR products were digested with several restriction enzymes and the resulted fragments were analysed using gel electrophoresis. The obtained results indicated nine alleles of HLA-G in Iranian individuals including G*01011 (4%), G*01012 (29.86%), G*01013 (10.8%), G*01015 (1.47%), G*01017 (1.96%), G*01018 (2.45%), G*01041 (29.4%), G*01043 (1.96%) and the null allele G* 0105N (18.1%). According to this study, in the Iranian subjects the most incident alleles were G*01012 and G*01041. The results for the frequency of G*01012 showed some similarity with Caucasians (36.3%).
机译：人白细胞抗原-G（HLA-G）是非经典HLA I类分子，与经典HLA不同，它具有低多态性。该分子最初在侵袭性滋养细胞上发现，并被认为在母胎界面中起中介作用。迄今为止，已发现43种HLA-G基因等位基因。 HLA-G基因等位基因的研究可能有助于理解伊朗人群中HLA-G等位基因的遗传变异。这项研究的目的是确定伊朗健康人群中HLA-G基因的多态性。使用盐析技术，然后通过HLA-G基因第2和第3外显子的聚合酶链反应（PCR）扩增，从102名随机选择，健康，无关的伊朗个体的全血中分离基因组DNA。为了执行PCR限制性片段长度多态性分析方法，用几种限制性酶消化PCR产物，并使用凝胶电泳分析所得片段。获得的结果表明伊朗个体中的HLA-G的9个等位基因包括G * 01011（4％），G * 01012（29.86％），G * 01013（10.8％），G * 01015（1.47％），G * 01017（ 1.96％），G * 01018（2.45％），G * 01041（29.4％），G * 01043（1.96％）和无效等位基因G * 0105N（18.1％）。根据这项研究，在伊朗受试者中，最常见的等位基因是G * 01012和G * 01041。 G * 01012的频率结果与白种人（36.3％）相似。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号