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首页> 外文期刊>International journal of immunogenetics >Dendritic cells transfected with lentiviral vector-encoding human granulocyte-macrophage colony-stimulating factor augment anti-tumour T-cell response in vitro
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Dendritic cells transfected with lentiviral vector-encoding human granulocyte-macrophage colony-stimulating factor augment anti-tumour T-cell response in vitro

机译：慢病毒载体转染编码人粒细胞巨噬细胞集落刺激因子的树突状细胞在体外增强抗肿瘤T细胞反应

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摘要

Dendritic cells (DC) are professional antigen-presenting cells that can actively taken up and present tumour-derived proteins to induce a tumour-specific immune response. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays a pivotal role in the generation, sensitization, maturation and survival of DC. We charged the peripheral blood monocyte cell-derived DC with tumour lysate, and then transfected the DC with lentiviral vector-encoding human GM-CSF (hGM-CSF). The antigen-presenting capacity of the hGM-CSF-transfected DC was tested by means of the mixed lymphocyte reaction and cytotoxic T-lymphocyte assay using wild-type DC as the control. The Lenti-hGM-CSF-transfected DC was able to stimulate the proliferation of naive allogeneic T lymphocytes and to generate tumour-specific cytotoxic T lymphocytes more efficiently than the wild-type DC. This data indicates that Lenti-hGM-CSF-transfected DC could potentially be used as an effective clinical approach for cancer immunotherapy.
机译：树突状细胞（DC）是可以主动摄取并呈递肿瘤衍生蛋白以诱导肿瘤特异性免疫反应的专业抗原呈递细胞。粒细胞巨噬细胞集落刺激因子（GM-CSF）在DC的产生，致敏，成熟和存活中起着关键作用。我们给外周血单核细胞来源的DC注入了肿瘤裂解物，然后用编码人GM-CSF（hGM-CSF）的慢病毒载体转染DC。 hGM-CSF转染的DC的抗原呈递能力通过混合淋巴细胞反应和以野生型DC为对照的细胞毒性T淋巴细胞测定法进行测试。慢病毒-hGM-CSF转染的DC能够刺激幼稚的同种T淋巴细胞的增殖，并且比野生型DC更有效地产生肿瘤特异性的细胞毒性T淋巴细胞。该数据表明，慢病毒-hGM-CSF转染的DC可以潜在地用作癌症免疫疗法的有效临床方法。

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