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首页> 外文期刊>International journal of immunogenetics >Full-length sequence and expression analysis of a myeloid differentiation factor 88 (MyD88) in half-smooth tongue sole Cynoglossus semilaevis.
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Full-length sequence and expression analysis of a myeloid differentiation factor 88 (MyD88) in half-smooth tongue sole Cynoglossus semilaevis.

机译：半光滑舌根半滑舌藻的髓样分化因子88（MyD88）的全长序列和表达分析。

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摘要

Myeloid differentiation factor 88 (MyD88) is a universal and crucial adaptor protein, which plays an essential role in the intracellular signalling elicited by IL-1R/TLR superfamily. In the present study, we report the full-length sequence of MyD88 gene in half-smooth tongue sole (Cynoglossus semilaevis). In the 2855 bp genomic sequence, five exons and four introns were identified. The cloned cDNA exhibited 110 bp of 5' UTR, 576 bp of 3' UTR and 858 bp of the entire open-reading frame encoding a polypeptide of 285 amino acids. The protein sequence included a typical conserved cytosolic Toll/interleukin-1 receptor (TIR) domain, an intermediate domain (ID) and a death domain (DD), and shared greater than 70% identity with Japanese flounder Paralichthys olivaceu ortholog. Real-time polymerase chain reaction (RT-PCR) analysis indicated a broad expression of csMyD88, especially in ovary and spleen. Quantitative RT-PCR analysis indicated that the csMyD88 mRNA levels were significantly increased in the spleen and head kidney after inactive Vibrio anguillarum challenge and the expression of csMyD88 appeared to be developmentally regulated during C. semilaevis ontogeny. Although, species-specific differences were present, the similarity between mammalian and piscine MyD88s suggested that the main function of MyD88 might be conserved across vertebrates.
机译：髓样分化因子88（MyD88）是一种通用且至关重要的衔接蛋白，在IL-1R / TLR超家族引发的细胞内信号传导中起着至关重要的作用。在本研究中，我们报告了MyD88基因在半光滑舌根（Cynoglossus semilaevis）中的全长序列。在2855 bp的基因组序列中，鉴定出5个外显子和4个内含子。克隆的cDNA展示了110 bp的5'UTR，576 bp的3'UTR和整个可读框的858 bp，编码285个氨基酸的多肽。蛋白质序列包括一个典型的保守的胞质Toll /白介素1受体（TIR）结构域，一个中间结构域（ID）和一个死亡结构域（DD），并且与日本比目鱼Paralichthys olivaceu ortholog具有超过70％的同一性。实时聚合酶链反应（RT-PCR）分析表明csMyD88广泛表达，尤其是在卵巢和脾脏中。定量RT-PCR分析表明，无活性的鳗弧菌攻击后，脾脏和头部肾脏中的csMyD88 mRNA水平显着增加，并且在半裸梭状芽孢杆菌发生期间csMyD88的表达受到发育调节。尽管存在物种特异性差异，但哺乳动物和鱼类MyD88之间的相似性表明，MyD88的主要功能可能在整个脊椎动物中得以保留。

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