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首页> 外文期刊>International journal of medical microbiology: IJMM >Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease
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Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease

机译：肠道菌群组成失衡可能与炎症性肠病的发病机制有关

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Since genetically engineered animal models of inflammatory bowel disease (IBD) do not develop colitis under germfree conditions, the intestinal microflora is thought to be one of the most important environmental factors associated with IBD. To understand the involvement of intestinal microflora, in the pathogenesis of IBD, we analyzed the constituents of intestinal microflora in IBD. Faecal samples from 73 patients with ulcerative colitis (UC) and 23 patients with Crohn's disease (CD) were analyzed by quantitative PCR using 16S rRNA gene-targeted group-specific primers for Bacteroides fragilis group, Bifidobacterium, Clostridium coccoides groups, Clostridium leptum subgroup, Atopobium cluster, and seven species of Bacteroides. We analyzed the distribution of the predominant microflora by fluorescence in situ hybridization (FISH) using group-specific probes. We also examined the concentration of faecal organic acids produced by intestinal microflora. Contrary to previous reports, we found that the B. fragilis group was significantly decreased in the faeces of patients with IBD. Moreover, R vulgatus was the predominant microflora in healthy controls and relatively decreased among IBD patients. Most of the microflora adhering to the colonic mucosa surrounding the mucus layer comprised C coccoides group and Bifidobacterium. B. fragilis group mainly inhabited the faeces, but did not adhere to or invade the mucosa. The concentrations of propionic and butyric acids in the faeces were significantly decreased in patients with IBD. These findings indicate that IBD is not caused by a specific intestinal bacterial cluster or species and that disordered intestinal microflora could be involved in the pathogenesis of IBD.
机译：由于炎性肠病（IBD）的基因工程动物模型在无菌条件下不会发展为结肠炎，因此肠道菌群被认为是与IBD相关的最重要的环境因素之一。为了了解肠道​​菌群参与IBD的发病机理，我们分析了IBD中肠道菌群的组成。使用16S rRNA基因靶向组特异性引物针对脆弱的拟杆菌，双歧杆菌，球状梭状芽胞杆菌组，梭状芽胞杆菌亚组，拟南芥簇生，和七种拟杆菌。我们使用组特异性探针通过荧光原位杂交（FISH）分析了主要菌群的分布。我们还检查了肠道菌群产生的粪便有机酸的浓度。与以前的报告相反，我们发现IBD患者的粪便中脆弱的芽孢杆菌组明显减少。此外，在健康对照组中，寻常性紫癜是最主要的菌群，在IBD患者中相对减少。粘附在粘液层周围的结肠粘膜上的大多数微生物群包括Ccoccoides基团和双歧杆菌。脆弱的芽孢杆菌群主要居住在粪便中，但没有粘附或侵入粘膜。 IBD患者的粪便中丙酸和丁酸的浓度明显降低。这些发现表明IBD不是由特定的肠道细菌簇或物种引起的，并且紊乱的肠道菌群可能与IBD的发病机理有关。

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