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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >RBE of alpha-particles from (211)At for complex DNA damage and cell survival in relation to cell cycle position.
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RBE of alpha-particles from (211)At for complex DNA damage and cell survival in relation to cell cycle position.

机译：来自（211）At的α粒子的RBE，涉及复杂的DNA损伤和与细胞周期位置有关的细胞存活率。

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摘要

PURPOSE: To investigate cell cycle effects and relative biological effectiveness (RBE) of alpha-particles from the clinically relevant radionuclide Astatine-211 ((211)At), using X-rays as reference radiation. Double-strand breaks (DSB), non-DSB clusters containing oxidised purines and clonogenic survival were investigated. MATERIALS AND METHODS: Asynchronous V79-379A fibroblasts or cells synchronised with mimosine in G1, early, mid and late S phase or in mitosis were irradiated with X-rays (100 kV(p)) or (211)At (mean linear energy transfer (LET) 110 keV/mum). Induction of DSB and clusters was determined using pulsed-field gel electrophoresis with fragment analysis. Cell survival was obtained with the clonogenic assay. RESULTS: In asynchronous cells RBE for DSB- and cluster-induction was 3.5 and 0.59, respectively. RBE for 37% cell survival was 8.6. In different cell cycle phases RBE varied from 1.8-3.9 for DSB and 3.1-7.9 for 37% survival (survival at 2 Gy was 6.9-38 times lower after alpha-irradiation). (211)At induced 6 times more DSB and X-rays induced 11 times more DSB in mitotic cells with highly compacted chromatin relative G1. CONCLUSIONS: The radio-response is cell cycle dependent and differs between proliferating and non-cycling cells for both low- and high-LET radiation, resulting in a variation in RBE of alpha-particles between 1.8 and 8.6.
机译：目的：使用X射线作为参考辐射，研究临床相关放射性核素A-211（（211）At）的α-颗粒的细胞周期效应和相对生物学有效性（RBE）。研究了双链断裂（DSB），含有氧化嘌呤的非DSB簇和克隆形成存活。材料与方法：用X射线（100 kV（p））或（211）At（平均线性能量转移）照射G1，早期，中期和晚期S期或有丝分裂中与含羞草同步的V79-379A异步成纤维细胞或细胞（LET）110 keV /妈妈）。使用脉冲场凝胶电泳和片段分析确定DSB和簇的诱导。通过克隆形成测定获得细胞存活。结果：在异步单元中，用于DSB和簇诱导的RBE分别为3.5和0.59。 37％细胞存活率的RBE为8.6。在不同的细胞周期阶段，RBE的DSB值在1.8-3.9之间，而37％的存活率在3.1-7.9之间变化（2 Gy的存活率是α照射后的6.9-38倍）。 （211）在染色质相对G1高度致密的有丝分裂细胞中，DSB诱导的DSB升高6倍，X射线诱导的DSB升高11倍。结论：放射性反应是细胞周期依赖性的，低和高LET辐射的增殖细胞和非循环细胞之间存在差异，导致α粒子的RBE在1.8和8.6之间变化。

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