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首页> 外文期刊>International Journal of Radiation Biology: Covering the Physical, Chemical, Biological, and Medical Effects of Ionizing and Non-ionizing Radiations >Radiation-induced up-regulation of telomerase in KG1a cells is influenced by dose-rate and radiation quality.
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Radiation-induced up-regulation of telomerase in KG1a cells is influenced by dose-rate and radiation quality.

机译：辐射诱导的KG1a细胞端粒酶的上调受剂量率和辐射质量的影响。

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摘要

Purpose: To examine the influence of dose, dose-rate and radiation quality on telomerase activity (TA) in the KG1a hematopoietic cell line. Materials and methods: KG1a cells were irradiated with gamma-rays (0.5-5 Gy) at 0.025 Gy/min, 0.30 Gy/min and 1.57 Gy/min and with a neutron/gamma-ray field (5 Gy). Cell viability was determined by trypan blue exclusion. Apoptosis and cell cycle distribution were evaluated by flow cytometry. Proliferative capacity was studied by MTS assay and TA by PCR. Following 3Gy gamma-irradiation, the expression of hTERT, hTR and TP1 genes was evaluated by RT-PCR. Results: Dose- and dose-rate-dependent telomerase activation with an increase in hTERT mRNA and a drop in hTP1 mRNA were observed after irradiation. Down-regulation of telomerase activity occurred in a dose-dependent manner. Although non-significant changes in short-term survival were observed after irradiation, late apoptosis became evident after G2/M arrest. Early repression of TA preceded telomerase activation in samples irradiated with a neutron/gamma-ray field, in which short-term survival was affected. Conclusions: Radiation-induced telomerase activation depends on dose-rate. High-LET and low-LET irradiations induce similar changes in TA that differ mainly in their kinetics and their magnitude. Changes in TA are not related to cell-cycle redistribution nor to the induction of cell death; they are the consequence of specific regulatory responses to ionizing radiation. Mechanisms including both transcriptional and post-translational control may be involved in this regulation.
机译：目的：研究剂量，剂量率和辐射质量对KG1a造血细胞系端粒酶活性（TA）的影响。材料和方法：用0.025 Gy / min，0.30 Gy / min和1.57 Gy / min的γ射线（0.5-5 Gy）和中子/γ射线场（5 Gy）照射KG1a细胞。通过锥虫蓝排除法测定细胞活力。通过流式细胞术评估细胞凋亡和细胞周期分布。通过MTS分析研究增殖能力，通过PCR研究TA。在3Gyγ射线照射后，通过RT-PCR评估了hTERT，hTR和TP1基因的表达。结果：辐射后观察到剂量和剂量率依赖性端粒酶激活，hTERT mRNA升高，hTP1 mRNA降低。端粒酶活性的下调以剂量依赖性方式发生。尽管辐照后观察到短期存活率无显着变化，但G2 / M停滞后晚期细胞凋亡变得明显。在中子/伽马射线场辐照的样品中，TA的早期抑制先于端粒酶活化，其中短期存活受到影响。结论：辐射诱导的端粒酶激活取决于剂量率。高LET和低LET辐射会诱导TA发生相似的变化，主要是在动力学和强度上有所不同。 TA的变化与细胞周期的再分配或细胞死亡的诱导无关。它们是对电离辐射的特定调节响应的结果。包括转录和翻译后控制的机制都可能参与此调控。

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