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Single neuron transcriptome analysis can reveal more than cell type classification Does it matter if every neuron is unique?

机译:单神经元转录组分析可以揭示的不仅仅是细胞类型分类,是否每个神经元都是唯一的,这有关系吗?

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A recent single cell mRNA sequencing study by Dueck et al. compares neuronal transcriptomes to the transcriptomes of adipocytes and cardiomyocytes. Single cell omic approaches such as those used by the authors are at the leading edge of molecular and biophysical measurement. Many groups are currently employing single cell sequencing approaches to understand cellular heterogeneity in cancer and during normal development. These single cell approaches also are beginning to address long-standing questions regarding nervous system diversity. Beyond an innate interest in cataloging cell type diversityinthebrain, single cell neuronal diversity has important implications for neurotypic neural circuit function and for neurological disease. Herein, we review the authors methods and findings, which most notably include evidence of unique expression profiles in some single neurons.
机译:Dueck等人最近的单细胞mRNA测序研究。比较神经元转录组与脂肪细胞和心肌细胞的转录组。单细胞组学方法(如作者使用的方法)处于分子和生物物理测量的前沿。当前,许多小组正在采用单细胞测序方法来了解癌症和正常发育过程中的细胞异质性。这些单细胞方法也开始解决有关神经系统多样性的长期问题。除了对大脑中的细胞类型多样性进行分类外,单细胞神经元多样性对于神经型神经回路功能和神经系统疾病也具有重要意义。本文中,我们回顾了作者的方法和发现,其中最值得注意的是包括某些单个神经元中独特表达谱的证据。

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