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首页> 外文期刊>Biochemistry and molecular biology education >Programmed Lab Experiments for Biochemical Investigation of Quorum-Sensing Signal Molecules in Rhizospheric Soil Bacteria
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Programmed Lab Experiments for Biochemical Investigation of Quorum-Sensing Signal Molecules in Rhizospheric Soil Bacteria

机译：根际土壤细菌中群体感应信号分子生化研究的程序化实验室实验

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Biochemistry courses in the Department of Molecular Biology at the National University of Rio Cuarto, Argentina, are designed for undergraduate students in biology, microbiology, chemistry, agronomy, and veterinary medicine. Microbiology students typically have previous coursework in general, analytical, and organic chemistry. Programmed sequences of lab experiments allow these students to investigate biochemical problems whose solution is feasible within the context of their knowledge and experience. We previously designed and reported a programmed lab experiment that familiarizes microbiology students with techniques for detection and characterization of quorum-sensing (QS) and quorum-quenching (QQ) signal molecules. Here, we describe a sequence of experiments designed to expand the understanding and capabilities of biochemistry students using techniques for extraction and identification of QS and QQ signal molecules from peanut rhizospheric soil bacteria, including culturing and manipulation of bacteria under sterile conditions. The program provides students with an opportunity to perform useful assays, draw conclusions from their results, and discuss possible extensions of the study. (C) 2016 by The International Union of Biochemistry and Molecular Biology, 44:256-262, 2016.
机译：阿根廷国立里约热内卢国立大学分子生物学系的生物化学课程是为生物学，微生物学，化学，农学和兽医学的本科生设计的。微生物学专业的学生通常在通用化学，分析化学和有机化学方面都有过以前的课程。通过编程的实验室实验序列，这些学生可以研究可在其知识和经验范围内解决问题的生化问题。我们之前设计并报告了一个程序化的实验室实验，该实验使微生物学的学生熟悉用于检测和表征群体感应（QS）和群体猝灭（QQ）信号分子的技术。在这里，我们描述了一系列实验，这些实验旨在利用从花生根际土壤细菌中提取和鉴定QS和QQ信号分子的技术来扩展生化专业学生的理解和能力，包括在无菌条件下培养和操作细菌。该计划为学生提供了进行有用的测定，从结果中得出结论以及讨论研究可能扩展的机会。 （C）2016年，国际生物化学与分子生物学联合会，44：256-262，2016。

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