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首页> 外文期刊>Biochemical Genetics >Increase in UV mutagenesis by heat stress on UV-irradiated E. coli cells.
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Increase in UV mutagenesis by heat stress on UV-irradiated E. coli cells.

机译：由于紫外线照射的大肠杆菌细胞上的热应激，紫外线诱变增加。

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摘要

When leu- auxotrophs of Escherichia coli, after UV irradiation, were grown at temperatures between 30 and 47°C, the frequency of UV-induced mutation from leu- to leu+ revertant increased as the UV dose and the temperature increased. For cells exposed to a UV dose of 45 J/m2, the mutation frequency at 47°C was 1.9 times that at 30°C; for a dose of 90 J/m2, it was 3.25 times; and for 135 J/m2, it was 4.8 times. Similar enhancement of reversion frequency was observed when the irradiated cells were grown at 30°C in the presence of a heat shock inducer, ethanol (8% v/v). Heat shock-mediated enhancement of UV mutagenesis did not occur in an E. coli mutant sigma 32 (heat shock regulator protein), but sigma 32 overexpression in the mutant strain (transformed with a sigma 32-bearing plasmid) increased the UV-induced mutation frequency. These results suggest that heat stress alone has no mutagenic property, but when applied to UV-damaged cells, it enhances the UV-induced frequency of cell mutation.
机译：紫外线照射后，当大肠杆菌的营养缺陷型细菌在30至47°C的温度下生长时，紫外线诱导的从leu-向leu +回复突变的频率随紫外线剂量和温度的升高而增加。对于暴露于45 J / m2紫外线的细胞，在47°C时的突变频率是30°C时的1.9倍;剂量为90 J / m2时，是3.25倍;对于135 J / m2，是4.8倍。当辐照的细胞在热激诱导剂乙醇（8％v / v）的存在下于30°C生长时，观察到相似的回复频率增强。在大肠杆菌突变体sigma 32（热激调节蛋白）中并未发生热激介导的UV诱变增强，但是在突变菌株（用带有sigma 32的质粒转化）中sigma 32过表达增加了UV诱导的突变。频率。这些结果表明，单独的热应激没有诱变特性，但是当应用于紫外线损伤的细胞时，它会增强紫外线诱导的细胞突变频率。

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