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首页> 外文期刊>Insect Molecular Biology >A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix
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A chitin deacetylase and putative insect intestinal lipases are components of the Mamestra configurata (Lepidoptera: Noctuidae) peritrophic matrix

机译:甲壳素脱乙酰酶和推定的昆虫肠道脂肪酶是Mamestra configurata(鳞翅目:夜蛾科)周围营养基质的组成部分

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摘要

One- and two-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify cDNA encoding a chitin deacetylase (McCDA1) and three insect intestinal lipases (McIIL1, McIIL2 and McIIL3) associated with the Mamestra configurata (bertha armyworm) peritrophic matrix. Recombinant McCDA1 was active and chitin deacetylase activities were detected in the midgut. McCDA1 and the McIIL genes were expressed exclusively in the midgut; however, McCDA1 and McIIL2 were expressed in all larval stages, whereas McIIL1 was expressed mainly in feeding larvae and McIIL3 primarily during the moult.
机译:一维和二维凝胶电泳结合液相色谱-串联质谱(LC-MS / MS)用于鉴定编码甲壳素脱乙酰基酶(McCDA1)和三种昆虫肠道脂肪酶(McIIL1,McIIL2和McIIL3)的cDNA。 Mamestra configurata(伯莎夜蛾)肥厚基质。重组McDA1是活跃的,并且在中肠中检测到了几丁质脱乙酰酶活性。 McCDA1和McIIL基因仅在中肠表达;然而,McDA1和McIIL2在幼虫的所有阶段均表达,而McIIL1主要在换羽期的幼虫和McIIL3的表达中表达。

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