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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system.
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Separation of dansylated amino acid enantiomers by chiral ligand-exchange CE with a zinc(II) L-arginine complex as the selecting system.

机译：以锌（II）L-精氨酸络合物为选择体系，通过手性配体交换CE分离丹磺酰化氨基酸对映体。

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摘要

A facile chiral ligand-exchange capillary electrophoretic method has been explored for the enantioseparation and UV detection of dansyl-amino acids with Zn(II) L-arginine complex as a chiral selecting system. Successful enantioseparation of 17 pairs of amino acid enantiomers has been achieved with a buffer of 100 mM boric acid, 5 mM ammonium acetate, 3 mM ZnSO(4) and 6 mM L-Arg at pH 8.0, of which 10 pairs were fully resolved with resolution in between 1.59 and 4.21. This new method was shown to be applicable to the separation of some mixed pairs of amino acids and to the quantitative analysis of some real samples such as rice vinegars, with a linear range between 0.8 and 150 mug/mL, correlation coefficient above 0.99 and recovery in between 90.1 and 112.4%. It was found that amino acids with low resistance side chain(s), low tendency to form intramolecular hydrogen bond or high tendency to form intermolecular hydrogen bonds are more easily enantioseparated than those with extra carboxyl and/or phenyl groups. By the use of the suggested buffer, the running pH should be selected at 7.4-9.0 to compromise the resolution and elution speed.
机译：探索了一种简便的手性配体交换毛细管电泳方法，用于手性选择系统，用于Zn（II）L-精氨酸配合物的对映体分离和紫外检测。用100 mM硼酸，5 mM乙酸铵，3 mM ZnSO（4）和6 mM L-Arg在pH 8.0的缓冲液中成功实现了17对氨基酸对映体的对映分离分辨率在1.59和4.21之间结果表明，该新方法适用于一些氨基酸混合对的分离，以及一些真实样品（例如米醋）的定量分析，线性范围在0.8至150杯/毫升之间，相关系数在0.99以上，并且回收率高。在90.1至112.4％之间。发现具有低电阻侧链，低形成分子内氢键的趋势或高形成分子间氢键的氨基酸比具有额外的羧基和/或苯基的氨基酸更容易对映分离。通过使用建议的缓冲液，应在7.4-9.0之间选择运行pH值，以降低分离度和洗脱速度。

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