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首页> 外文期刊>Electrophoresis: The Official Journal of the International Electrophoresis Society >Alternative sample preparation prior to two-dimensional electrophoresis protein analysis on solid lipid nanoparticles
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Alternative sample preparation prior to two-dimensional electrophoresis protein analysis on solid lipid nanoparticles

机译：在固体脂质纳米颗粒上进行二维电泳蛋白质分析之前的替代样品制备

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The proteins adsorbing onto the surface of intravenously injected drug carriers are regarded as a key factor determining the organ distribution. Depending on the particle surface properties, certain proteins will be preferentially adsorbed, leading to the adherence of the particle to cells with the appropriate receptor. Therefore, the knowledge of the protein adsorption pattern and the correlation to in vivo behavior opens the perspective for the development of intravenous colloidal carriers for drug targeting. After incubation in plasma, the adsorbed proteins were analyzed using two-dimensional polyacrylamide gel electrophoresesis (2-D PAGE, 2-DE). The purpose of the present study was to develop an alternative separation method to separate solid lipid nanoparticles (SLN) carriers from plasma by gel filtration prior to 2-D PAGE. Via the specific absorption coefficients and a two-equation system, elution fractions were identified being practically plasma-free. This allows protein analysis on SLN which are typically in density too close to the density value of water to be separated by the standard centrifugation method. The SLN used for establishing the gel filtration were prepared in a way that they had a sufficiently low density to be additionally separated by centrifugation. The adsorption patterns obtained after separation with both methods were qualitatively and quantitatively identical, showing the suitability of the gel filtration. [References: 39]
机译：吸附在静脉注射药物载体表面的蛋白质被认为是决定器官分布的关键因素。根据颗粒的表面性质，某些蛋白质将被优先吸附，从而导致颗粒与具有适当受体的细胞粘附。因此，对蛋白质吸附模式及其与体内行为的相关性的了解为开发用于药物靶向的静脉内胶体载体开辟了前景。在血浆中孵育后，使用二维聚丙烯酰胺凝胶电泳（2-D PAGE，2-DE）分析吸附的蛋白质。本研究的目的是开发另一种分离方法，以在2-D PAGE之前通过凝胶过滤从血浆中分离固体脂质纳米颗粒（SLN）载体。通过比吸收系数和两方程系统，可以确定洗脱级分几乎没有血浆。这允许对SLN进行蛋白质分析，该蛋白质的密度通常太接近水的密度值，无法通过标准离心方法进行分离。用于建立凝胶过滤的SLN的制备方法是，使它们具有足够低的密度以通过离心另外分离。两种方法分离后获得的吸附模式在质量和数量上均相同，显示了凝胶过滤的适用性。 [参考：39]

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