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Carbon nanotube composite as novel platform for microbial biosensor

机译：碳纳米管复合材料作为微生物生物传感器的新型平台

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摘要

The presented work includes the development of a microbial biosensor based on a carbon-nanotube epoxy composite (CNTEC) platform used as supporting electrode for cell immobilization. For this purpose, cells of Pseudomonas fluorescens were immobilized on the surface of the CNTEC electrode by means of gelatin which was then cross linked with glutaraldehyde. After optimization of experimental parameters like cell amount, pH and temperature, the system was calibrated for glucose. From the calibration graph the linear range was estimated as 0.5 - 4.0 mM with a response time of 100 s. Furthermore, substrate specificity and operational stability were investigated. Finally, the results that were obtained with CNTEC electrodes were compared with conventional graphite epoxy composite electrode (GECE) and as a result, higher current values (2 to 3 folds) were observed with CNTEC microbial biosensor.
机译：提出的工作包括基于碳纳米管环氧复合材料（CNTEC）平台的微生物生物传感器的开发，该平台用作细胞固定的支撑电极。为此，通过明胶将荧光假单胞菌的细胞固定在CNTEC电极的表面上，然后将其与戊二醛交联。优化实验参数（如细胞量，pH和温度）后，对系统进行葡萄糖校准。根据校准曲线，线性范围估计为0.5-4.0 mM，响应时间为100 s。此外，研究了底物特异性和操作稳定性。最后，将CNTEC电极获得的结果与常规石墨环氧复合电极（GECE）进行了比较，结果，使用CNTEC微生物生物传感器观察到了更高的电流值（2至3倍）。

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