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首页> 外文期刊>Electroanalysis >Differential pulse voltammetric determination and application of square-wave voltammetry of yRNA on a CPB-Cellulose modified electrode
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Differential pulse voltammetric determination and application of square-wave voltammetry of yRNA on a CPB-Cellulose modified electrode

机译：CPB-纤维素修饰电极上的差分脉冲伏安法测定yRNA的方波伏安法及其应用

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摘要

Electrochemical behavior of remarkably low levels of Ribonucleic acid yeast (yRNA) is studied through differential pulse voltammetry (DPV), and kinetic parameters of the electrochemical reaction have also been calculated through square-wave voltammetry (SWV), based on immobilization of yRNA on the surface of a CPB-cellulose modified electrode. YRNA/ CPB-cetlulose/ITO conductive glass electrode is demonstrated by Infrared reflect (IR) and electrochemical impedance spectroscopy (EIS). The oxidation peak potential of yRNA shifts negatively with increasing pH. ne peak currents decrease gradually in successive scans and no corresponding reduction peaks occur, indicating that oxidation process of yRNA is completely irreversible. Variables influencing DPV response of yRNA, such as pH, pulse amplitude and electrolyte concentration, are explored and optimized. Peak currents are proportional to the concentration of yRNA in the range of 0.1 mu g mL(-1)-1.0 mu g mL(-1), and the linear regression coefficient equals 0.9923. The detection limit for yRNA is 1.0 x 10(-10) g mL(-1). Interferences of L-cysteine, L-alanine, Hemoglobin, Uridine 5 '-monophosphate, Guanosine 5 '-monophosphate, Adenosine 5 '-triphosphate and some metal ions (Co3+, Cr3+, Ni2+ Hg2+, Zn2+, etc) are negligible. The methods adopted here are more sensitive and selective than currently applied techniques and overcome the drawback of absorption spectroscopy arising from a strong interference due to other UV-absorbing substances.
机译：通过差示脉冲伏安法（DPV）研究了极低水平的核糖核酸酵母（yRNA）的电化学行为，并且基于yRNA固定在电池上的方波伏安法（SWV）也计算了电化学反应的动力学参数。 CPB纤维素修饰电极的表面。 YRNA / CPB-纤维素/ ITO导电玻璃电极通过红外反射（IR）和电化学阻抗谱（EIS）证明。 yRNA的氧化峰电位随着pH的增加而负向移动。在连续扫描中，峰值电流逐渐降低，并且没有相应的还原峰出现，表明yRNA的氧化过程是完全不可逆的。探索和优化了影响yRNA的DPV响应的变量，例如pH，脉冲幅度和电解质浓度。峰值电流与yRNA的浓度成正比，范围为0.1μg mL（-1）-1.0μg mL（-1），线性回归系数等于0.9923。 yRNA的检出限为1.0 x 10（-10）g mL（-1）。 L-半胱氨酸，L-丙氨酸，血红蛋白，尿苷5'-单磷酸酯，鸟苷5'-单磷酸酯，腺苷5'-三磷酸酯和某些金属离子（Co3 +，Cr3 +，Ni2 + Hg2 +，Zn2 +等）的干扰可忽略不计。与目前应用的技术相比，此处采用的方法更加灵敏和更具选择性，并且克服了由于其他紫外线吸收物质引起的强干扰而导致的吸收光谱法的缺点。

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