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首页> 外文期刊>Equine and Comparative Exercise Physiology >Effects of intra-articular hyaluronic acid on ex vivo responses by third carpal bone cartilage from horses with experimentally induced synovitis.
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Effects of intra-articular hyaluronic acid on ex vivo responses by third carpal bone cartilage from horses with experimentally induced synovitis.

机译：关节内透明质酸对实验性滑膜炎马的第三腕骨软骨离体反应的影响。

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摘要

We studied the extent to which intra-articular hyaluronic acid (HA) administered to horses with experimentally induced synovitis in the third middle carpal joint altered cartilage proteoglycans (PGs) and the biomechanical properties of the third carpal bone. Intra-articular HA (Hylartin-VReg.) was administered to the middle carpal joints of healthy horses before and after the induction of synovitis by Escherichia coli lipopolysaccharide (LPS). Two groups of horses received intra-articular HA only, with evaluation 2 and 6 weeks later (group 2 and group 4). Two other groups (group 1 and group 3) received LPS followed by intra-articular HA and evaluation 2 and 6 weeks later. Ex vivo third carpal bone cartilage PG synthesis was measured by 35SO4 incorporation and third carpal bone cartilage PG content was determined by an assay for glucuronic acid. Biomechanical properties were measured by indentation testing. At the 2-week evaluation, HA did not ablate the quantitative reduction in PG synthesis and PG content in animals with experimental synovitis. However, by 6 weeks, there was a significant rebound effect in that PG synthesis and PG content were elevated in horses with experimental synovitis that had received intra-articular HA, compared with horses that had received HA only for that period of time. There were also alterations in the gel filtration profiles of newly synthesized PGs on Sepharose CL-2B among the groups. However, there were no de novo PG species associated with any of the treatments. Furthermore, we found that the biomechanical properties of third carpal bone cartilage were not altered in any of the groups. These studies showed that intra-articular HA administered to horses with experimentally induced synovitis did not provide short-term (i.e. 2-week) benefit to third carpal bone cartilage PG synthesis. However, a long-term benefit of intra-articular HA may occur after the acute effects of synovitis on PG synthesis and PG content subside.
机译：我们研究了在第三中腕关节中对实验性滑膜炎的马施用关节内透明质酸（HA）的程度改变了软骨蛋白聚糖（PGs）的含量以及第三腕骨的生物力学特性。在大肠杆菌脂多糖（LPS）诱发滑膜炎之前和之后，对健康马的中腕关节给予关节内HA（Hylartin-VReg。）。两组马只接受了关节内HA，并在2周和6周后进行了评估（第2组和第4组）。另外两个组（第1组和第3组）接受LPS，随后进行关节内HA评估，并在2周和6周后进行评估。通过35SO 4掺入测量离体第三腕骨软骨PG的合成，并通过对葡萄糖醛酸的测定来确定第三腕骨软骨PG的含量。通过压痕测试测量生物力学性能。在2周的评估中，HA并未消除实验性滑膜炎动物体内PG合成和PG含量的定量减少。然而，到6周时，与仅接受HA的马相比，接受关节内HA的实验性滑膜炎马的PG合成和PG含量增加，具有明显的反弹作用。各组之间在Sepharose CL-2B上新合成的PG的凝胶过滤特性也发生了变化。然而，没有与任何治疗相关的从头PG物质。此外，我们发现第三组腕骨软骨的生物力学特性在任何组中均未改变。这些研究表明，向患有实验性滑膜炎的马施用关节内HA不能为第三腕骨软骨PG合成提供短期（即2周）的益处。然而，滑膜炎对PG合成和PG含量的急性影响减弱后，可能会产生关节内HA的长期益处。

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