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首页> 外文期刊>Enzyme and Microbial Technology >Production of cellulose and hemicellulose-degrading enzymes by filamentous fungi cultivated on wet-oxidised wheat straw
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Production of cellulose and hemicellulose-degrading enzymes by filamentous fungi cultivated on wet-oxidised wheat straw

机译：湿氧化小麦秸秆上培养的丝状真菌生产纤维素和半纤维素降解酶

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摘要

The production of cellulose and hemicellulose-degrading enzymes by cultivation of Aspergillus niger ATCC 9029, Botrytis cinerea ATCC 28466, Penicillium brasilianum IBT 20888, Schizophyllum commune ATCC 38548, and Trichoderma reesei Rut-C30 was studied. Wet-oxidised wheat straw suspension supplemented with NH4NO3, MgSO4, and KH2PO4 was used as cultivation medium aiming to obtain an enzyme mixture optimal for enzymatic hydrolysis of wet-oxidised wheat straw. The cultivations with B. cinerea and R brasilianum gave the highest endoglucanase (EC 3.2.1.4) and beta-glucosidase (EC 3.2.1.21) activities, in contrast to the other fungi where lower activities were found. The culture filtrates were concentrated by ammonium sulphate precipitation. After enzyme concentration, the highest enzyme activities (1.34 FPU/ml) were found in the culture broth originating from P. brasilianum. Enzymatic hydrolysis of filter cake from wet-oxidised wheat straw for 48 h with an enzyme loading of 5 FPU/g biomass resulted in glucose yields from cellulose of 58% (w/w) and 39% (w/w) using enzymes produced by R brasilianum and a commercial enzyme mixture, respectively. At higher enzyme loading (25 FPU/g biomass) using either enzyme mixtures the glucose yield from cellulose was in the range of 77-79% (w/w).
机译：研究了通过培养黑曲霉ATCC 9029，灰霉菌ATCC 28466，巴西青霉IBT 20888，裂褶菌公社ATCC 38548和里氏木霉Rut-C30来生产纤维素和半纤维素降解酶。以补充有NH4NO3，MgSO4和KH2PO4的湿氧化麦草悬浮液为培养基，旨在获得最适合于酶法水解湿氧化麦草的酶混合物。与发现较低活性的其他真菌相反，用灰质芽孢杆菌和巴西利亚霉的培养物产生最高的内切葡聚糖酶（EC 3.2.1.4）和β-葡萄糖苷酶（EC 3.2.1.21）活性。通过硫酸铵沉淀浓缩培养物滤液。酶浓缩后，在源自巴西假单胞菌的培养液中发现最高的酶活性（1.34 FPU / ml）。湿法氧化小麦秸秆滤饼经过48 h的酶水解，酶负荷为5 FPU / g生物量，使用由以下方法制得的酶，纤维素的葡萄糖得率分别为58％（w / w）和39％（w / w）。巴西利亚芥和商业酶混合物。使用任何一种酶混合物，在较高的酶负载下（25 FPU / g生物质），纤维素的葡萄糖产率为77-79％（w / w）。

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