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首页> 外文期刊>Enzyme and Microbial Technology >Production of cellulose and hemicellulose-degrading enzymes by filamentous fungi cultivated on wet-oxidised wheat straw
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Production of cellulose and hemicellulose-degrading enzymes by filamentous fungi cultivated on wet-oxidised wheat straw

机译：湿氧化小麦秸秆上培养的丝状真菌生产纤维素和半纤维素降解酶

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摘要

The production of cellulose and hemicellulose-degrading enzymes by cultivation of Aspergillus niger ATCC 9029,Botrytis cinerea ATCC 28466,Penicllium brasilianum IBT 20888,Schizophyllum commune ATCC 38548,and Trchoderma ressei Rut-C30 was studied.Wet-ocidised wheat straw suspension supplemented with NH_4NO_3,MgSO_4,and KH_2PO_4 was used as cultivation medium aiming to obtain an enzyme nixture optimal for enzymatic hydrolysis of wet-oxidised wheat straw.The cultivation with B.cincrea and P.brasilianum game the highest endogulucanase(EC3.2.1.4)and beta-glucosidase (EC3.2.1.21)activities,in contrast to the other fungi where lower activities were found.The culture filtrates were concentrated by ammonium suplhate precipitation.After enzyme concentration,the highest enzyme activites (1.34FPU/ml)were found in the culture broth originating from p.brasilianum.Enzymatic hydrolysis of filter cake from wet-oxidised wheat straw for 48 h with an enzyme loading of 5FPU/gbiomass resulted in glucode yields from cellulose of 58%(w/w)and 39%(w/w)using enzymes produced by P.brasilianum and a commercial enzyme mixture,respectively.At higher enzyme loading (25FPU/g biomass)using either enzyme mixtures the glucose yield from cellulose was in the range of 77-79%(w/w).
机译：研究了黑曲霉ATCC 9029，灰葡萄孢菌ATCC 28466，巴西青霉IBT 20888，裂褶菌公社ATCC 38548和水生木霉菌Rut-C30的发酵生产纤维素和半纤维素的酶。 ，MgSO_4和KH_2PO_4作为培养基，旨在获得最适合湿法氧化小麦秸秆酶水解的酶。 -葡糖苷酶（EC3.2.1.21）活性，而其他真菌的活性较低。通过硫酸铵沉淀浓缩培养液。酶浓缩后，最高酶活性为（1.34FPU / ml）。来自湿氧化麦草的滤饼经酶水解5FPU /生物量负载的湿氧化麦草滤饼发酵48小时，产生了葡萄糖分别使用巴西利亚假单胞菌产生的酶和商业酶混合物从58％（w / w）和39％（w / w）的纤维素中获得的lds。在较高的酶负载下（25FPU / g生物量），使用任一种酶混合物的葡萄糖纤维素的收率在77-79％（w / w）的范围内。

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