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首页> 外文期刊>Brachytherapy >Comparison of cellular damage response to low-dose-rate 125I seed irradiation and high-dose-rate gamma irradiation in human lung cancer cells
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Comparison of cellular damage response to low-dose-rate 125I seed irradiation and high-dose-rate gamma irradiation in human lung cancer cells

机译：低剂量率125I种子辐射和高剂量率γ辐射对人肺癌细胞的细胞损伤反应比较

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Purpose: To investigate the difference of cellular response between low-dose-rate (LDR) 125I seed irradiation and high-dose-rate (HDR) γ-irradiation in human lung cancer cells. Methods and Materials: A549 and NCI-H446 cells with or without wortmannin (WM) treatment were exposed to 125I seeds and γ-rays, respectively. Cell survival, micronuclei (MN) formation, and the expressions of Ku70/Ku80 proteins were measured. Results: There was a strong negative correlation between survival and MN formation for both irradiations, and the MN inductions of NCI-H446 were about twofolds of those of A549, and the survival of NCI-H446 was lower than that of A549, indicating the radiosensitivity of NCI-H446 cells was greater than that of A549 cells. Interestingly, at 4-Gy radiation, NCI-H446 cells were more sensitive to LDR irradiation than HDR irradiation. WM treatment enhanced the radiosensitivity of A549 cells evenly to 125I seed and γ-irradiation, but this treatment led NCI-H446 cells to be more sensitive to LDR 125I. Further results revealed that the expression of phosphorylated Ku80 protein was enhanced in irradiated A549, but in contrast, it was markedly decreased in NCI-H446 cells after 4-Gy LDR 125I irradiation as that compared with γ-irradiated and nonirradiated cells. Conclusion: NCI-H446 cells were more sensitive to LDR 125I irradiation than HDR irradiation, and this sensitivity could be further enhanced by WM treatment. But no obvious differences of cellular response to both irradiations were observed in A549. Ku as molecular markers together with cell proliferation rate can be used to predict the radiosensitivity of tumor cells to LDR 125I seed irradiation.
机译：目的：研究低剂量率（LDR）125I种子辐照与高剂量率（HDR）γ辐照对人肺癌细胞的细胞应答差异。方法和材料：将经过或未经过渥曼青霉素（WM）处理的A549和NCI-H446细胞分别暴露于125I种子和γ射线。测量细胞存活，微核（MN）形成和Ku70 / Ku80蛋白的表达。结果：两种照射的存活率和MN形成之间均存在很强的负相关性，NCI-H446的MN诱导约为A549的两倍，NCI-H446的存活率低于A549，表明放射敏感性NCI-H446细胞的数量大于A549细胞的数量。有趣的是，在4-Gy辐射下，NCI-H446细胞对LDR辐射比对HDR辐射更敏感。 WM处理均匀地增强了A549细胞对125I种子和γ射线的放射敏感性，但是这种处理导致NCI-H446细胞对LDR 125I更敏感。进一步的结果表明，磷酸化的Ku80蛋白的表达在被辐照的A549中得到增强，但相比之下，在4-Gy LDR 125I辐照后的NCI-H446细胞中，其表达明显低于被γ辐照和未辐照的细胞。结论：NCI-H446细胞对LDR 125I辐射比对HDR辐射更敏感，这种敏感性可以通过WM处理进一步增强。但是在A549中没有观察到细胞对两种照射的反应的明显差异。 Ku作为分子标记以及细胞增殖速率可用于预测肿瘤细胞对LDR 125I种子辐射的放射敏感性。

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