...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>European journal of nutrition >Short-chain fatty acid (SCFA) uptake into Caco-2 Cells by a pH-dependent and carrier mediated transport mechanism
【24h】

Short-chain fatty acid (SCFA) uptake into Caco-2 Cells by a pH-dependent and carrier mediated transport mechanism

机译：pH依赖性和载体介导的转运机制将短链脂肪酸（SCFA）吸收到Caco-2细胞中

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The short-chain fatty acids, acetate, propionate, and butyrate, are the most abundant organic anions in the human colon. SCFA play a pivotal role in maintaining homeostasis in the colon. Particularly butyrate induces cell differentiation and regulates growth and proliferation of colonic mucosal epithelial cells, whereas it reduces the growth rate of colorectal cancer cell. Previous studies by several groups. including our own, using isolated membrane vesicles have demonstrated that the uptake of butyrate is at least in part mediated by a non-electrogenic SCFA-/HCO_3~- antiporter. The purpose of the present study was to determine (1) whether Caco-2 cells could serve as an experimental model to assess the mechanisms of SCFA transport, and (2) whether monocar-boxlate transporters could play a role in SCFA transport in these cells. Caco-2 cells were found to transport ~14C-butyrate in a concentration and time dependent manner. The uptake was sodium independent, but was stimulated by lowering extracellular pH. The uptake of 500#mu#M butyrate was reduced by 49.6% +- 3.3% in the presence of propionate and by 57.2% +- 4.8% in the presence of 10 mML-lactate. The addition of 1 mM #alpha#-cyano-4-hydroxycinnamate and phloretin, both known to be potent inhibitors of MCT1, decreased the uptake of 500 #mu#M ~14C-butyrate by 59.4% +- 4.1% and 48.9% +- 3.3%, respectively, whereas similar concentrations of DIDS did not have any effect. These data suggest that the up-take of butyrate in Caco-2 cells ocurs via a carrier mediated transport system specific for monocar-boxylic acidsm, which is in accordance with characteristics of the MCT 1.
机译：短链脂肪酸乙酸盐，丙酸盐和丁酸盐是人类结肠中最丰富的有机阴离子。 SCFA在维持结肠内稳态方面起着关键作用。特别地，丁酸盐诱导细胞分化并调节结肠粘膜上皮细胞的生长和增殖，而它降低结直肠癌细胞的生长速率。以前的研究由几个小组进行。包括我们自己在内，使用分离的膜囊泡已证明丁酸的吸收至少部分地由非电SCFA- / HCO_3〜-反转运蛋白介导。本研究的目的是确定（1）Caco-2细胞是否可以用作评估SCFA转运机制的实验模型，以及（2）单羧酸盐转运蛋白是否可以在这些细胞中的SCFA转运中发挥作用。发现Caco-2细胞以浓度和时间依赖性方式转运〜14C丁酸酯。摄取与钠无关，但通过降低细胞外pH刺激。在丙酸酯存在下，500＃mu＃M丁酸酯的摄取降低了49.6％±3.3％，在10 mML乳酸酯存在下降低了57.2％±4.8％。加入已知是MCT1的有效抑制剂的1 mM＃alpha＃-氰基-4-羟基肉桂酸酯和促视紫红质，可使500＃mu＃M〜14C丁酸酯的吸收量降低59.4％+-4.1％和48.9％+ -分别为3.3％，而类似浓度的DIDS没有任何作用。这些数据表明，Caco-2细胞中丁酸酯的摄取是通过特定于一元羧酸的载体介导的转运系统发生的，这符合MCT 1的特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号