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首页> 外文期刊>European journal of nutrition >Modulation of CYP19 expression by cabbage juices and their active components: indole-3-carbinol and 3,3'-Diindolylmethene in human breast epithelial cell lines.
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Modulation of CYP19 expression by cabbage juices and their active components: indole-3-carbinol and 3,3'-Diindolylmethene in human breast epithelial cell lines.

机译：甘蓝汁及其活性成分：人乳腺上皮细胞系中吲哚-3-甲醇和3,3'-二吲哚基亚甲基对CYP19表达的调节。

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Purpose: The aim of this study was to evaluate the effect of white cabbage and sauerkraut juices of different origin and indole-3-carbinol (I3C) and diindolylmethane (DIM) on expression of CYP19 gene encoding aromatase, the key enzyme of estrogen synthesis. Methods: Human breast cell lines (MCF7, MDA-MB-231 and MCF10A) were examined to compare the action of cabbage juices versus their active components (I3C, DIM). Real-time PCR and Western blot were used in order to analyse CYP19 mRNA and protein, respectively. Results: Remarkable differences in the effect on CYP19 transcript and protein level were found between the cabbage juices (in 2.5-25 mL/L concentrations) and indoles (in 2.5-50 micro M doses) in the three cell lines. While cabbage juices at the lower doses diminished the aromatase expression in nontumorigenic/immortalized MCF10A breast cells (0.25-0.86-fold change, P<0.05), I3C and DIM were more efficient in decreasing the aromatase expression in estrogen-dependant MCF7 breast cancer cells (0.24-0.82-fold change, P<0.05). Inhibition of aromatase by juice obtained from cabbage grown on industrial farm was correlated with the induction of apoptosis (1.7-1.8-fold change, P<0.01) in MCF10A cells. In estrogen-independent MDA-MB-231 cells, up-regulation of CYP19 expression by I3C and DIM (1.5-2.0-fold change, P<0.05) was observed. Similarly, in MCF7 cells juices increased aromatase expression (1.1-2.2-fold change, P<0.05). Conclusion: These results, particularly that obtained in nontumorigenic/immortalized MCF10A cells, suggest that chemopreventive activity of cabbage against breast cancer observed in epidemiological studies may be partly explained by inhibition of the aromatase expression.
机译：目的：本研究的目的是评估不同来源的白菜和酸菜汁以及吲哚-3-甲醇（I3C）和二吲哚基甲烷（DIM）对编码芳香化酶（是雌激素合成关键酶）的CYP19基因表达的影响。方法：检查人乳腺细胞系（MCF7，MDA-MB-231和MCF10A），以比较白菜汁与其活性成分（I3C，DIM）的作用。为了分别分析CYP19的mRNA和蛋白，使用了实时PCR和Western blot。结果：在三种细胞系的白菜汁（浓度为2.5-25 mL / L）和吲哚（浓度为2.5-50 microM）之间，对CYP19转录本和蛋白质水平的影响存在显着差异。低剂量白菜汁减少了非致瘤/永生化MCF10A乳腺癌细胞中的芳香化酶表达（变化0.25-0.86倍，P <0.05），I3C和DIM在降低雌激素依赖性MCF7乳腺癌细胞中的芳香化酶表达方面更有效。 （0.24-0.82倍变化，P ＜0.05）。工业农场种植的白菜汁对芳香酶的抑制作用与诱导MCF10A细胞凋亡（1.7-1.8倍变化，P <0.01）有关。在不依赖雌激素的MDA-MB-231细胞中，观察到I3C和DIM对CYP19表达的上调（变化为1.5-2.0倍，P <0.05）。同样，在MCF7细胞中，汁液增加了芳香化酶的表达（1.1-2.2倍变化，P <0.05）。结论：这些结果，特别是在非致​​瘤性/永生化MCF10A细胞中获得的结果，表明在流行病学研究中观察到的白菜对乳腺癌的化学预防活性可能部分是由于抑制了芳香化酶的表达。

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