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首页> 外文期刊>European food research and technology =: Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A >Optimization of an enzymatic wheat gluten hydrolysis process in an enzyme membrane reactor using a design of experiment approach
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Optimization of an enzymatic wheat gluten hydrolysis process in an enzyme membrane reactor using a design of experiment approach

机译：实验方法的设计优化酶膜反应器中小麦面筋的水解工艺

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The aim of this study was to determine the optimal conditions for operating an enzyme membrane reactor system to obtain a high yield of amino acids and peptides released from wheat gluten with Flavourzyme (TM). The optimal operating conditions were determined using multivariate analysis. A fractional factorial design made up of 27 runs amended by eight axial points was followed in a bench-scale enzyme membrane reactor system (V = 2 L), and responses were recorded over 8 h. Optimal conditions determined were a temperature of 50 +/- A 1 A degrees C and a Flavourzyme (TM) activity of 116 +/- A 4 nkat(Leu-pNA)/mL. Modeling resulted in an optimal substrate feed rate of 19.2 g/L/h and a permeate flux of 57 L/m(2)/h. The enzymatic wheat gluten hydrolysis in an enzyme membrane reactor was conducted for 72 h with an average total product space-time yield of 12.6 +/- A 1.3 g/L/h (n = 4) and a free amino groups space-time yield of 4.4 +/- A 0.4 g/L/h (n = 4), and the substrate utilization was 0.82 +/- A 0.01 (g/g) (n = 4). The resulting product-enzyme ratios were 7.7 +/- A 0.6 mg(TP)kat(Leu-pNA) (n = 4) and 3.0 +/- A 0.2 mg(FAA)kat(Leu-pNA) (n = 4). The productivity of the enzyme membrane reactor improved compared to the reference batch process and doubled compared to previously proposed enzyme membrane reactor processes for the hydrolysis of wheat gluten.
机译：这项研究的目的是确定操作酶膜反应器系统的最佳条件，以使用Flavourzyme（TM）从小麦面筋中获得高产量的氨基酸和多肽。使用多变量分析确定最佳运行条件。在台式规模的酶膜反应器系统（V = 2 L）中遵循由8个轴向点修改的27个运行组成的分数阶乘设计，并在8小时内记录响应。确定的最佳条件是温度为50 +/- A 1 A摄氏度，Flavourzyme（TM）活性为116 +/- A 4 nkat（Leu-pNA）/ mL。建模后的最佳底物进料速度为19.2 g / L / h，渗透流量为57 L / m（2）/ h。在酶膜反应器中进行的小麦面筋水解反应进行了72小时，平均总时空产率为12.6 +/- A 1.3 g / L / h（n = 4），游离氨基时空产率值为4.4 +/- A 0.4 g / L / h（n = 4），底物利用率为0.82 +/- A 0.01（g / g）（n = 4）。生成的产物-酶比为7.7 +/- A 0.6 mg（TP）/ nkat（Leu-pNA）（n = 4）和3.0 +/- A 0.2 mg（FAA）/ nkat（Leu-pNA）（n = 4）。与参考间歇方法相比，酶膜反应器的生产率提高了，与先前提出的用于水解小麦面筋的酶膜反应器方法相比，其生产率提高了一倍。

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