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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >Assembly of fission yeast eisosomes in the plasma membrane of budding yeast: Import of foreign membrane microdomains
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Assembly of fission yeast eisosomes in the plasma membrane of budding yeast: Import of foreign membrane microdomains

机译：在发芽酵母的质膜中组装裂变酵母酶体：导入外来膜微区

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摘要

Eisosomes are plasma membrane-associated protein complexes organizing the membrane compartment of Can1 (MCC), a membrane microdomain of specific structure and function in ascomycetous fungi. By heterologous expression of specific components of Schizosaccharomyces pombe eisosomes in Saccharomyces cerevisiae we reconstitute structures exhibiting the composition and morphology of S. pombe eisosome in the host plasma membrane. We show S. pombe protein Pil1 (SpPil1) to substitute the function of its S. cerevisiae homologue in building plasma membrane-associated assemblies recognized by inherent MCC/eisosome constituents Sur7 and Seg1. Our data indicate that binding of SpPil1 to the plasma membrane of S. cerevisiae also induces formation of furrow-like invaginations characteristic for MCC. To the best of our knowledge, this is the first report of interspecies transfer of a functional plasma membrane microdomain. In the described system, we identify a striking difference between eisosome stabilizer proteins Seg1 and SpSle1. While Seg1 recruits both Pil1 and SpPil1 to the plasma membrane, SpSle1 recognizes only its natural counterpart, SpPil1. In the presence of Pil1, SpSle1 is segregated outside the Pil1-organized eisosomes and forms independent microdomains in the host membrane. (C) 2014 Elsevier GmbH. All rights reserved.
机译：脂质体是组织Can1（MCC）的膜区室的血浆膜相关蛋白复合物，MC1是子囊性真菌中特定结构和功能的膜微区。通过在酿酒酵母中异源表达粟酒裂殖酵母的特定成分，我们重构了在宿主质膜中展现粟酒裂殖酵母的组成和形态的结构。我们显示S. pombe蛋白Pil1（SpPil1）替代其啤酒酵母同系物的功能，以构建由固有MCC /酶体成分Sur7和Seg1识别的质膜相关组件。我们的数据表明，SpPil1与酿酒酵母质膜的结合也诱导了MCC的沟状侵入现象的形成。据我们所知，这是功能性质膜微区间种间转移的首次报道。在描述的系统中，我们确定了异构体稳定蛋白Seg1和SpSle1之间的显着差异。虽然Seg1将Pil1和SpPil1都募集到质膜上，但SpSle1仅识别其天然对应物SpPil1。在存在Pil1的情况下，SpSle1被隔离在Pil1组成的Esosome之外，并在宿主膜中形成独立的微区。 （C）2014 Elsevier GmbH。版权所有。

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