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首页> 外文期刊>European Journal of Cell Biology: Journal of Deutsche Gesellschaft fur Elektronenmikroskopie: Journal of Deutsche Gesellschaft fur Zellbiologie >RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila
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RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila

机译：需要RNA解旋酶Spn-E来维持果蝇种系中piRNA沉默的Aub和AGO3蛋白水平

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摘要

Germline-specific RNA helicase Spindle-E (Spn-E) is known to be essential for piRNA silencing in Drosophila that takes place mainly in the perinuclear nuage granules. Loss-of-function spn-E mutations lead to tandem Stellate genes derepression in the testes and retrotransposon mobilization in the ovaries. However, Spn-E functions in the piRNA pathway are still obscure. Analysis of total library of short RNAs from the testes of spn-E heterozygous flies revealed the presence of abundant piRNA ping-pong pairs originating from Su(Ste) transcripts. The abundance of these ping-pong pairs were sharply reduced in the library from the testes of spn-E mutants. Thus we found that ping-pong mechanism contributed to Su(Ste) piRNA generation in the testes. The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules. We found that observed decline of the protein expression was not caused by suppression of aub and ago3 transcription as well as total transcription, indicating possible contribution of Spn-E to post-transcriptional regulation. (C) 2016 Elsevier GmbH. All rights reserved.
机译：已知种系特异性RNA解旋酶Spindle-E（Spn-E）对于果蝇中的piRNA沉默至关重要，而果蝇中的piRNA沉默主要发生在核周成核颗粒中。功能丧失的spn-E突变导致睾丸中的串联Stellate基因表达降低，卵巢中的反转录转座子动员。然而，在piRNA途径中Spn-E的功能仍然不清楚。分析来自spn-E杂合蝇的睾丸的短RNA的总文库，发现存在大量源自Su（Ste）转录本的piRNA乒乓球对。从库中的spn-E突变体的睾丸中大量减少这些乒乓球。因此，我们发现乒乓机制有助于睾丸中Su（Ste）piRNA的产生。 Spn-E的缺乏导致雄性和雌性种系中主要的乒乓参与者，PIWI亚科的茄子（Aub）和AGO3蛋白的蛋白质水平显着下降，但并未破坏它们在成粒颗粒中的组装。我们发现观察到的蛋白表达下降不是由抑制aub和ago3转录以及总转录引起的，这表明Spn-E对转录后调控的可能贡献。 （C）2016 Elsevier GmbH。版权所有。

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