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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
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DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.

机译：DEC-205 / CD205 +树突状细胞在人脾的白色牙髓中丰富，包括红色和白色牙髓之间的边界区域。

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The distribution of dendritic cells (DCs) and macrophages in the human spleen has received less attention than that of lymphocytes. Here we have addressed this problem with the human DEC-205/CD205 marker ('DEC'), which is an endocytic receptor on DCs that mediates efficient presentation of antigens. DEC was abundant on dendritic profiles in the white pulp but absent from the red pulp, the latter defined with antibodies to two antigens, mannose receptor/CD206 on sinusoidal lining cells, and macrosialin/CD68 on macrophages. Double staining with anti-DEC and anti-CD3 showed the expected concentration of DEC+ cells in the relatively small T-cell areas of the human spleen. DEC+ cells were also found in other regions of the white pulp. In all regions, the DEC+ cells were positive for major histocompatibility complex (MHC) class II and the CD11c integrin but largely immature, with low expression of B7-2/CD86 costimulator and DC-lysosome-associated membrane protein (LAMP)/CD208. When we concentrated on the perifollicular region between the red pulp and the marginal zone, we found macrophages that stained with antibodies to sialoadhesin/CD169 and DC-specific ICAM-3 grabbing non-integrin (SIGN)/CD209, and just inside these cells were DEC+ profiles. The DEC+ DCs were intertwined with cells that stained for the vascular addressin mucosal addressin cell adhesion molecule (MAdCAM). Therefore, anti-DEC-205/CD205 antibodies are useful for identifying DCs in human splenic white pulp and its border region with the red pulp.
机译：树突状细胞（DC）和巨噬细胞在人脾脏中的分布受到的关注少于淋巴细胞。在这里，我们已经用人DEC-205 / CD205标记（'DEC'）解决了这个问题，该标记是DC上的一种内吞受体，介导抗原的有效呈递。 DEC在白色果肉的树突状分布中含量丰富，但在红色果肉中则不存在，后者用两种抗原的抗体定义：正弦内衬细胞上的甘露糖受体/ CD206和巨噬细胞上的大唾液酸蛋白/ CD68。用抗DEC和抗CD3双重染色显示了人脾中相对较小的T细胞区域中DEC +细胞的预期浓度。在白色果肉的其他区域也发现了DEC +细胞。在所有地区，DEC +细胞均对主要的组织相容性复合物（MHC）II类和CD11c整联蛋白呈阳性，但未成熟，B7-2 / CD86共刺激物和DC溶酶体相关膜蛋白（LAMP）/ CD208的表达较低。当我们集中在红髓与边缘区之间的滤泡周围区域时，我们发现巨噬细胞被抗唾液酸粘附素/ CD169和DC特异性ICAM-3捕获非整联蛋白（SIGN）/ CD209的抗体染色，并且这些细胞内部DEC +个人资料。 DEC + DC与缠绕了血管膜粘膜寻址蛋白细胞粘附分子（MAdCAM）的血管膜交织在一起。因此，抗DEC-205 / CD205抗体可用于鉴定人脾白浆及其与红浆的边界区域中的DC。

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